基于Arduino及其外围传感器控制高压脉冲发生器用于细胞不可逆电穿孔实验

Control of High Voltage Pulse Generator Based on Arduino and Its Peripheral Sensors for Cell Irreversible Electroporation Test

提出一种基于Arduino及其外围脉搏、温度传感器控制高压脉冲发生器的方案用于细胞不可逆电穿孔实验。通过对Arduino开发板编程,将脉搏、温度传感器采集的模拟量光电信号和热敏电阻分压信号转换为数字量信号和温度数值。设定心电R波阈值(>550)和温度阈值(<37 ℃)作为触发条件,以此控制一台功率800W的高压脉冲发生器释放一次固定周期的脉冲。应用体外培养的人肺癌细胞测试验证,细胞染色评估穿孔情况。结果表明,Arduino及其传感器触发和反馈灵敏,控制高压脉冲发生器释放100个电压600 V、场强1 200 V/cm、脉宽100 μs的脉冲条件下,95%以上的细胞出现了非热性的不可逆电穿孔。

A project of control of a high voltage pulse generator based on Arduino and its peripheral pulse sensor and temperature sensor was proposed for cell irreversible electroporation(IRE) test. By programming Arduino board, the analog photoelectric signal and the partial voltage signal of thermistor collected by pulse and temperature sensors were converted into digital signal and temperature value. The threshold of ECG R wave(>550) and temperature threshold(<37 ℃) was set as trigger condition to control an 800 W high voltage pulse generator to release a fixed period pulse. Human lung cancer cells cultured in vitro were used to test and verify, and cell staining was used to evaluate the perforation. The results showed that Arduino and its sensors were sensitive to trigger and feedback. When the high voltage pulse generator was controlled to release 100 pulses with the parameters of 600 V, 1 200 V/cm and 100 μs pulse width, more than 95% of the cells showed nonthermal irreversible electroporation.