摘要
为确诊一例七彩山鸡疑似感染寄生虫死亡的原因,使用剖检、光镜形态学检测、HE病理染色观察等方法进行了临床诊断,使用PCR技术对获得的虫体18S rRNA进行扩增,鉴定感染虫体类型。剖检发现,病禽肠道黏膜内有细长白条状的线虫体寄生,感染数量较多,肝、心等部位未见明显病变;形态学检测发现,该线虫具有较为典型的尾刺结构。结合剖检症状和形态学鉴定,初步判断病禽感染的消化道线虫为异刺线虫。病理组织学检查发现,虫体寄生的肠道黏膜出现损伤及炎性症状;分子生物学鉴定发现,该线虫18S rRNA的PCR扩增条带大小为760 bp,同源性与鸡异刺线虫(DQ503462.1)最接近。经综合诊断,判定异刺线虫感染是导致本次七彩山鸡死亡的主要原因。本次分析鉴定的虫株与家禽源异刺线虫同源性较高,表明七彩山鸡感染的异刺线虫可能来自鸡等普通家禽。这提示,散养特种禽应定期驱虫,加强禽舍卫生清扫,尽量避免与普通家禽混养。
It was reported that several Phasianus colchicus suspected of being infected with parasites died,in order to confirm the cause of death,the Phasianus colchicus were clinically diagnosed through autopsy,morphological examination by light microscope,and observation by HE pathological staining,then for the obtained worm,18 S rRNA was amplified by PCR to identify the type of parasite.It was found that,by autopsy,the intestinal mucosae of the Phasianus colchicus were infested by a large number of long white-striped nematodes,and no obvious lesions were observed in livers and hearts;it was found that,by morphological examination,the nematodes were with typical tail spines.It was preliminarily determined that what the Phasianus colchicus infected was Heterakis according to the symptoms as observed by autopsy and morphological examination.It was found that,by histopathological examination,the intestinal mucosae where worms parasitized were damaged with inflammatory symptoms;it was found that,by molecular biological identification,the amplified band of 18 S rRNA was 760 bp,its homology was closest to that of Heterorhabdus gallinarum(DQ503462.1).It was concluded that the death of the Phasianus colchicus was mainly caused by Heterakis infection.The homology of the isolates identified in the study with poultry Heterakis was closest,which indicated that the Heterakis in Phasianus colchicus might come from chicken and other common poultry.Therefore,special poultry kept in free range should be regularly dewormed,their houses should be thoroughly cleaned and any mixed feeding with common poultry should be avoided as far as possible.
作者
黄潇航
彭佳佳
李诗艺
贺金峪
王诗晨
黄志坚
黄瑜
殷光文
Huang Xiaohang;Peng Jiajia;Li Shiyi;He Jinyu;Wang Shichen;Huang Zhijian;Huang Yu;Yin Guangwen(College of Animal Sciences of Fujian Agriculture and Forestry University,Fujian Enginecring Laboratory of Animal Pharmaceuticals,Fuzhou,Fujian 350002,China;Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China)
出处
《中国动物检疫》
CAS
2021年第8期95-98,102,共5页
China Animal Health Inspection
基金
国家自然基金项目(31872466)
福建农林大学优秀硕士基金项目(1122YS01004)
福建农林大学科技创新基金项目(CXZX2020125B,CXZX2019070G)
福建省禽病防治重点实验室课题资助项目(FKADL-2019-02)。
关键词
七彩山鸡
异刺线虫
临床诊断
分子生物学
Phasianus colchicus
Heterakis
clinical diagnosis
molecular biology