Endostatin、VEGF及FGF-2与喉鳞癌发生的相关性及其临床价值探讨

Relationship between endostatin,VEGF,and FGF-2 and the occurrence of laryngeal squamous cell carcinoma and their clinical value

目的探讨内皮抑素(Endostatin)、血管内皮生长因子(VEGF)及碱性成纤维细胞生长因子(FGF-2)在喉鳞癌患者中的表达及与疾病发生的相关性,及临床价值。方法选择59例喉鳞癌患者设为观察组,均经手术病理组织检查确诊,术中取病灶组织与癌旁组织(距肿瘤>5 cm);选择同期56例非喉部肿瘤手术治疗患者为对照组,取喉腔黏膜组织。采用免疫组织化学法完成两组Endostatin、VEGF及FGF-2水平测定;记录观察组病灶组织病理资料,分析不同临床特征下Endostatin、VEGF及FGF-2表达情况,采用Spearman相关性分析Endostatin、VEGF及FGF-2与喉鳞癌发生的相关性。结果观察组癌旁组织与对照组Endostatin、VEGF及FGF-2蛋白阳性率比较均无统计学意义(χ^(2)分别=1.79、2.08、1.43,P均>0.05);观察组病灶组织中Endostatin、VEGF及FGF-2蛋白阳性率均高于癌旁组织和对照组(χ^(2)分别=10.39、12.14、7.94;6.97、10.85、9.24,P均>0.05);免疫组化结果显示,观察组病灶组织中Endostatin、VEGF及FGF-2呈高表达,喉细胞出现不同程度坏死,纤维增生更加明显。Spearman相关性分析显示,喉鳞癌发生与Endostatin、VEGF及FGF-2蛋白表达呈正相关(rs分别=0.59、0.56、0.62,P均<0.05)。结论Endostatin、VEGF及FGF-2在喉鳞癌患者中呈高表达,能反映患者疾病严重程度,且与喉鳞癌的发生存在相关性,能为临床诊疗提供依据和参考。

Objective To investigate the expressions of endostatin,vascular endothelial growth factor(VEGF)and ba-sic fibroblast growth factor(FGF-2)in laryngeal squamous cell carcinoma(LSCC)and their correlation with the occur-rence of LSCC.Methods Totally 59 patients with LSCC who were confirmed by pathological examination were selected as the observation group,and the focus tissue and adjacent tissue(more than 5 cm away from the tumor)were taken dur-ing the operation.Totally 56 patients with non laryngeal tumor during the same period were selected as the control group,and the laryngeal mucosa tissue was taken.Immunohistochemistry was used to determine the levels of endostatin,VEGF and FGF-2 in the two groups.The histopathological data of lesions in the observation group were recorded,and the expres-sions of endostatin,VEGF and FGF-2 in different clinic characteristics were analyzed.Spearman correlation analysis was used to analyze the correlation between endostatin,VEGF and FGF-2 and the occurrence of laryngeal squamous cell car-cinoma.Results The positive rates of endostatin,VEGF,and FGF-2 proteins in the adjacent tissues of the observation group and the control group were not statistically significant(χ^(2)=1.79,2.08,1.43,P>0.05).The positive rates of en-dostatin,VEGF,and FGF-2 proteins in the lesion tissues of the observation group were higher than those of the adjacent tissues and the control group(χ^(2)=10.39,12.14,7.94,6.97,10.85,9.24,P>0.05).The results of immunohistochemistry showed that the endostatin,VEGF and FGF-2 were highly expressed in the lesion tissues of the observation group,and the laryngeal cells showed different degrees of necro-sis and fibrous hyperplasia.Spearman correlation analysis showed that the occurrence of laryngeal squamous cell carcinoma was positively correlated with the postive expressions of endostatin,VEGF,and FGF-2 proteins(rs=0.59,0.56,0.62,P<0.05).Conclusion Endostatin,VEGF,and FGF-2 are highly expressed in patients with laryngeal squamous cell carcinoma,which can reflect the severity of the disease.They have correlation with the occurrence of laryngeal squamous cell carcinoma,which can provide reference for clinical diagnosis and treatment.