基于微流控芯片的血脑屏障体外模型构建

Construction of An in Vitro Blood-brain Barrier Model Based on Microfluidic Chip

血脑屏障(BBB)介于血液和脑组织之间对物质转运具有选择性阻碍作用,并以此维持脑组织的营养物质运输和正常代谢。BBB结构的完整性与中枢神经系统(CNS)疾病的发生、发展、治疗、预后等密切相关。深入了解BBB的结构与功能有助于CNS疾病发生机制的研究及相关药物的研发。合适的BBB体外模型是此类研究的有效工具。近年来,随着微流控芯片技术的发展,基于微流控芯片技术的BBB研究被广泛的应用于生物和临床医学,特别是其可精确控制、检测和操纵细胞微环境的特点,为CNS疾病发生机制的研究及相关药物的研发提供了一种新的有力工具。本文将对基于微流控芯片的BBB体外模型构建及其应用进行总结,并对未来基于微流控芯片的BBB体外研究可能遇到的挑战进行探讨。

The blood-brain barrier(BBB)is located between the blood and brain tissue to selectively hinder the transport of substances,and to maintain the nutrient transport and normal metabolism of the brain tissue.The structural integrity of BBB is closely related to the occurrence,development,treatment,and prognosis of central nervous system(CNS)diseases.An in-depth understanding of the structure and function of BBB is helpful to the research on the mechanism of CNS disease and the development of related drugs.A suitable in vitro model of BBB is an effective tool for this type of research.In recent years,with the development of microfluidic chip technology,BBB research based on microfluidic chip technology has been widely used in biology and clinical medicine,especially its characteristics of precise control,detection and manipulation of the cellular microenvironment.It provides a new powerful tool for the research on the mechanism of CNS disease and the development of related drugs.This article will summarize the construction and application of BBB in vitro model based on microfluidic chip.And discuss the possible challenges of BBB in vitro research based on microfluidic chip in the future.