纤维脱胶菌RJ6的筛选及脱胶性能

Screening of Fiber Degumming Bacteria RJ6 and Its Degumming Performance

针对植物纤维因其纤长且坚韧在造纸和防治工业中应用受限的问题,从腐烂菜堆中筛选到分离得到纤维脱胶菌株RJ6,将该菌接种到以苎麻汁为唯一有机营养成分的培养基中对苎麻进行脱胶实验,对其进行了形态鉴定、生理生化特性实验以及16S rDNA序列的比对,并对菌株的发酵条件进行优化。测得接种RJ6菌株实验组相对于对照组的脱胶率达到91%,说明RJ6对植物纤维原料有高效的脱胶作用。对菌株的发酵条件进行优化,显示RJ6菌株在转速160 r/min、接种量5%、温度37℃和起始pH 7.0条件下保持一个高水平的发酵。形态鉴定、生理生化特性实验以及16S rDNA序列比对结果表明,RJ6为短杆状,两端钝圆,大小约为(0.4~0.5)μm×(1.1~1.8)μm,革兰氏染色结果呈阴性,存在端生鞭毛。其16S rDNA序列与GenBank中的果胶杆菌属(Pectobacterium.sp)同源性大于99%,RJ6属于果胶杆菌属(Pectobacterium.sp)。

Aiming at the issue of the limited use of plant fibers in paper making and control treatment industries due to their length and tenacity,the screening of fiber degumming bacteria RJ6 was isolated and screened from the rotting vegetable,and the strain was inoculated into a medium with ramie juice as the only organic nutrient component to perform degumming experiments on ramie.Morphological identification,physiological and biochemical characteristics and 16S rDNA sequence comparison were carried out,and the fermentation conditions of the strain were optimized.The results showed that the degumming rate of the experimental group inoculated with RJ6 was 91%,compared to the control group,indicating that RJ6 had an efficient degumming effect on plant fiber raw materials.By optimizing the fermentation conditions of strain RJ6,it was shown that the RJ6 maintained a high level of fermentation under the conditions of 160 r/min,5%inoculation,37℃and initial pH 7.The results of morphological identification,physiological and biochemical characteristics experiment,and 16S rDNA sequence alignment showed that RJ6 was short rod shaped,blunt round at both ends,approximately(0.4~0.5)μm×(1.1~1.8)μm in size.The test results were negative for Gram staining,and there were terminal flagella.The 16S rDNA sequence of RJ6 was more than 99%homology with Pectobacterium.sp from GenBank,and it also showed that RJ6 belonged to Pectobacterium.sp.