正交试验-多指标综合评分法优选小儿肠系膜淋巴结炎中药复方汤剂的水提工艺

Optimization of Water Extraction Process of the Chinese Medicine Compound Decoction for Mesenteric Lymphadenitis by Orthogonal Test and Multi-index Comprehensive Scoring

目的:基于正交试验-多指标综合评分法对小儿肠系膜淋巴结炎中药复方汤剂的水提工艺进行筛选、优化。方法:采用高效液相色谱法(HPLC),以吴茱萸碱、吴茱萸次碱、木香烃内酯、去氢木香烃内酯、桂皮醛和丁香酚为指标成分,以加水量、水提时间和提取次数为考察因素,采用单因素分析法、正交试验-多指标综合评分法加以评价。结果:该复方汤剂的最佳提取工艺为加水量8倍量,回流提取3次,每次1.5 h。结论:筛选的小儿肠系膜淋巴结炎中药复方汤剂的水提工艺稳定、可行,能够为临床确保该汤剂的质量提供参考。

Objective:To optimize the water extraction process of Chinese medicine compound decoction for mesenteric lymphadenitis based on orthogonal test and multi-index comprehensive scoring.Methods:High performance liquid chromatography(UPLC)was employed to determine the index components evodiamine,rutaecarpine,costundide,dehydrocostus lactone,cinnamaldehyde,and eugenol.Single-factor test,orthogonal test,and multi-index comprehensive scoring were utilized to determine the optimal extraction parameters of water-to-material ratio,extraction duration,and extraction times.Results:The optimized extraction parameters are as follows:Water-to-material ratio of 8 and 3 times of reflux extraction with 1.5 h/time.Conclusion:The optimized water extraction process of the compound decoction for mesenteric lymphadenitis in this experiment is stable and feasible,which can serve as a reference for quality control of the decoction in clinical practice.