摘要
目的探讨细胞周期蛋白L1(CCNL1)对人绒毛膜滋养细胞生物学行为的影响及调控机制。方法以人绒毛膜滋养细胞HTR⁃8/SVneo为模型,根据转染的类型分为CCNL1过表达组,过表达对照组,CCNL1干扰组1,CCNL1干扰组2,CCNL1干扰组3,干扰对照组,采用实时荧光定量(qPCR)及Western Blot方法检测CCNL1 mRNA和蛋白水平的表达变化;CCK⁃8法检测增殖能力;Transwell方法检测细胞侵袭、迁移能力;流式技术检测细胞周期和凋亡情况。结果过表达CCNL1后,滋养层细胞的迁移、侵袭能力降低(P<0.05);增殖能力降低(P<0.05);细胞周期S期的细胞数减少(P<0.05)。干扰CCNL1后,滋养层细胞的迁移、侵袭能力增强(P<0.05);增殖能力增强(P<0.05)。结论CCNL1基因可能通过调控滋养层细胞的迁移、侵袭以及增殖能力而影响胎盘的功能。
Objective To explore the effect of Cyclin L1(CCNL1)on the biological behaviors and regula⁃tory mechanism of human trophoblast cells.Methods We used HTR⁃8/SVneo as the model and divide it into CCNL1 overexpression group,CCCNL1 overexpression control group,CCNL1 interference group 1,CCNL1 inter⁃ference group 2,CCNL1 interference group 3 and interference control group.The mRNA expression level of CCNL1 was detected by Quantitative Real⁃time PCR(qPCR);the protein expression level of CCNL1 by Western Blot and cell proliferation by CCK⁃8.Cell migration and invasion were detected by Transwell assay and cell cycle and cell apoptosis by flow cytometry.Results Overexpression CCNL1 significantly decreased the migration and invasion ability of trophoblast cells(P<0.05),the proliferation ability of trophoblast cells(P<0.05),and the number of S cells(P<0.05).Interfered CCNL1 significantly increased the migration and invasion ability of tropho⁃blast cells(P<0.05),and the proliferation ability of trophoblast cells(P<0.05).Conclusion CCNL1 gene may affect placental function by regulating the migration,invasion and proliferation of trophoblast cells.
作者
杨闪闪
唐冉冉
江华
汤秋勤
YANG Shanshan;TANG Ranran;JI-ANG Hua;TANG Qiuqin(Nanjing Medical University,Nanjing 210029,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2021年第14期1789-1794,共6页
The Journal of Practical Medicine
基金
国家自然科学基金项目(编号:81771597,81971405)。