水稻基因OsATS的克隆及功能鉴定

Cloning and functional identification of gene OsATS in rice

植物胚胎特异性蛋白ATS3和植物的渗透胁迫响应有密切关系,本文对水稻OsATS基因的抗逆相关功能进行了初步研究。qRT-PCR检测发现,水稻在盐胁迫后OsATS基因表达量显著增加。构建OsATS基因过表达载体,转化拟南芥植株,抗逆性检测表明, OsATS基因的过表达可以显著提高拟南芥在萌发阶段和成株阶段的耐盐性。随后将过表达载体p1300-35S:OsATS和RNA干涉载体pTCK303-OsATS-RNAi转入水稻,抗逆性分析表明,OsATS过表达水稻株系在萌发阶段和苗期的耐盐性显著提高,而OsATS基因RNAi水稻株系耐盐性则明显下降。qRT-PCR和生理指标检测表明,OsATS基因的表达可能通过调节OsP5CS1、OsLEA3-1、OsPDH基因的表达,调控了水稻细胞中的脯氨酸、LEA蛋白质含量,进而影响了水稻植株整体的耐盐性。本研究初步揭示了OsATS基因的抗逆功能,后续可通过调整该基因的表达量,改良水稻的抗逆性。

The plant embryo specific protein ATS3 is closely related to osmotic stress response in plants.Here,the stress resistance related gene OsATS was preliminarily studied in rice.Fluorescence quantitative PCR showed that the relative expression level of OsATS increased significantly after salt stress in rice.The overexpression vector of OsATS was constructed and transformed into Arabidopsis thaliana.The stress resistance test revealed that the overexpression of OsATS gene could significantly improve the salt tolerance of Arabidopsis thaliana at germination and adult stages.After that,the overexpression vector p1300-35s:OSATS and RNA interference vector pTCK303-OsATS-RNAi were transferred into rice.The stress tolerance analysis indicated that the salt tolerance of OsATS overexpression rice lines significantly increased at germination stage and seedling stage,while the salt tolerance of OsATS RNAi rice lines significantly decreased.Results of qRT-PCR and physiological index detection demonstrated that the relative expression levels of OSATS gene might regulate the protein content of proline and LEA cells by regulating the expression of OsP5CS1,OsLEA3-1 and Os PDH,thus affecting the salt tolerance in rice.This study preliminarily revealed the stress resistance function of OSATS gene,which laid a foundation for improving rice stress resistance by adjusting the relative expression level of OSATS gene.