两种采样方法对大肠埃希菌噬菌体气溶胶采样效果的研究

Study on effect of two sampling methods for Coliphage aerosol collection

目的研究两种采样方法对大肠埃希菌噬菌体气溶胶采集的效果。方法采用固体撞击式采样法和液体撞击式采样法,比较两种空气微生物采样器对大肠埃希菌噬菌体气溶胶的采样效果。结果在相同气溶胶环境下,固体撞击式采样器采样结果,回收的噬菌体浓度平均为1.04×10^(5) pfu/m^(3);液体撞击式采样器采样结果,回收的噬菌体浓度平均为5.71×10^(5) pfu/m^(3)。大肠埃希菌噬菌体气溶胶粒径主要集中在0.653.3μm,噬菌斑计数时,是否进行阳性孔换算,其总数差异约2倍,但平均颗粒直径差异较小。气溶胶初始浓度为1.08×10^(5) pfu/m^(3),60 min自然消亡率为28.96%。结论大肠埃希菌噬菌体气溶胶具有较好的稳定性,固体撞击法和液体撞击法采集方法各有优缺点,可根据实际试验需求进行选择。

Objective To research the effect of two sampling methods on the collection of Coliphage aerosol.Methods The solid impact sampling method and the liquid impact sampling method were used to compare the sampling effects of two air microbial samplers on Escherichia coli bacteriophage aerosol.Results Under the same aerosol condition,the aerosol concentration was 1.04×10^(5) pfu/m^(3) recovered by the solid impact method,and 5.71×10^(5) pfu/m^(3) recovered by the liquid impact method.The main sizes of Coliphage aerosol particle was between 0.65~3.3 μm.After the conversion of postive pores,the total number of phage plaque was about 2 times,but the difference of average particle diameter was small.The initial aerosol concentration was 1.08×10^(5) pfu/m^(3),and the natural extinction rate was 28.96% in 60 min.Conclusion Coliphage aerosol has good stability,and its concentration collection methods of solid impact method and liquid impact method have their own advantages and disadvantages,which can be selected according to the actual experimental requirements.