深海真菌Neoroussoella sp.固体发酵物中次生代谢产物及其生物活性研究

Study on the secondary metabolites from the deep-sea-derived fungus Neoroussoella sp.and their biological activities

研究深海真菌Neoroussoella sp.FS526的次生代谢产物及生物活性。采用硅胶柱、Sephadex LH-20凝胶柱和高效液相等多种色谱技术对菌株FS526的固体发酵物中的次生代谢产物进行分离纯化,并通过NMR等波谱方法鉴定化合物结构。从菌株FS526中共分离鉴定了15个化合物,分别为amestolkolide B(1)、thailandolide B(2)、aspergillumarins A和B(3和4)、de-O-methyldiaporthin(5)、4-(2-acetoxyethyl)phenol(6)、5,6,8-三羟基-4-(1′-羟乙基)异香豆素(7)、sescandelin(8)、penicifuran A(9)、7-羟基-5-甲氧基-4,6-邻苯二甲酯(10)、pestalotiolactone A(11)、2′,4′-二羟基-6′-甲氧基-3′-甲基苯乙酮(12)、1-(2,4-二羟基-6-甲氧基-3-甲基苯基)乙酮(13)、2,5-二甲基间苯二酚(14)、3,5-二羟基甲苯(15)。所有化合物均为首次从该属真菌中分离得到。细胞毒和抗氧化活性测试表明化合物1具有较弱的细胞毒活性,其IC50值为41.44~77.89μM;化合物7具有较强的抗氧化活性,其IC50值为13.93μM。

The secondary metabolites of a deep-sea-derived fungus Neoroussoella sp.FS526 and their biological activities were investigated.The solid fermentation extraction of strain FS526 was isolated and purified by silica gel column,Sephadex LH-20 gel column,and high performance liquid chromatography,which led to the isolation of fifteen known compounds.The chemical structures of the isolates were identified by spectrum analysis as amestolkolide B(1),thailandolide B(2),aspergillumarins A-B(3 and 4),de-O-methyldiaporthin(5),4-(2-acetoxyethyl)phenol(6),5,6,8-trihydroxy-4-(1′-hydroxyethyl)isocoumarin(7),sescandelin(8),penicifuran A(9),7-hydroxy-5-methoxy-4,6-dimethylphthalidc(10),pestalotiolactone A(11),2′,4′-dihydroxy-6′-methoxy-3′-methylacetophenone(12),1-(2,4-dihydroxy-6-methoxy-3-methylphenyl)ethanone(13),2,5-dimethylresorcinol(14),and 3,5-dihydroxytoluene(15).All the isolated compounds were firstly reported from the genus Neoroussoella.Compound 1 showed weak cytotoxic activity with the IC50 values of 41.44-77.89μM,and compound 7 showed strong antioxidant activity with the IC50 value of 13.93μM.