蜂胶乙醇提取物对小鼠主动脉内皮细胞损伤的保护作用

Protective Effects of Propolis Ethanol Extract on Mouse Aortic Endothelial Cells Injury

目的:探究蜂胶乙醇提取物(ethanol extracts of propolis,EEP)对脂多糖(lipopolysaccharide,LPS)诱导的小鼠主动脉内皮细胞(mouse aortic endothelial cell,MAEC)炎症因子损伤的保护作用。方法:将细胞分为对照组,LPS模型组,蜂胶低(2.5μg/mL)、中(5μg/mL)、高(10μg/mL)剂量组。采用CCK-8检测MAEC的细胞增殖率,ELISA酶联免疫吸附实验测定MAEC炎症细胞中TNF-α、IL-6的含量,Western Blot法测定MAEC炎症细胞中ICAM-1、VCAM-1、MCP-1的表达水平。结果:与对照组相比,LPS组MAEC的细胞增殖率极其显著降低(P<0.001),ICAM-1、VCAM-1、MCP-1、TNF-α以及IL-6的水平极其显著升高(P<0.001)。经不同浓度EEP处理后,MAEC的细胞增殖率显著上升(P<0.05或P<0.01),TNF-α、IL-6的含量以及ICAM-1、VCAM1、MCP-1表达水平降低,各蜂胶组与LPS组相比均有显著性差异(P<0.01或P<0.001)。结论:EEP能够抑制LPS诱导的MAEC中炎症因子的表达,对血管内皮细胞具有保护作用。

Objective:The aim of this study was to investigate the protective effects of ethanol extracts of propolis(EEP)on mouse aortic endothelial cells(MAEC)from injury induced by lipopolysaccharide(LPS).Methods:Cell proliferation rate was determined by CCK-8.Then MAEC were divided into the blank group,LPS model group,low-dose(2.5μg/mL),middle-dose(5μg/mL),and high-dose(10μg/mL)groups of EEP.The TNF-αand IL-6 levels were evaluated by ELISA.The expression levels of ICAM-1,VCAM-1 and MCP-1 were measured by Western Blot.Results:Compared with the control group,the cell proliferation rate of MAEC in the LPS group was extremely significant reduced(P<0.001),and the levels of ICAM-1,VCAM-1,MCP-1,TNF-αand IL-6 were extremely significant increased(P<0.001).The cell proliferation rate of EEP groups increased significantly(P<0.05 or P<0.01),and the content of TNF-α,IL-6 and the expression levels of ICAM-1,VCAM-1,and MCP-1 decreased compared with these of LPS group.There were significant differences between each propolis group and LPS group(P<0.01 or P<0.001).Conclusion:EEP could effectively inhibit LPS-induced the expression of inflammatory factors in MAEC,and it has the effect of protecting endothelial cells.