花粉传播黄龙病可能性研究

Evaluation of the Possibility of Pollen Transmission of Huanglongbing caused by'Candidatus Liberibacter asiati-cus'

为明确柑橘黄龙病能否通过授粉途径进行传播,研究采集了黄龙病发病沙田柚的花粉,将其授到6株健康温州蜜柑树的花上,定期观察被授粉树症状,并通过实时荧光定量PCR方法检测是否感染黄龙病菌。结果表明,授粉后30 d、60 d、90 d和180 d,被授粉树均生长正常,未出现黄龙病症状,也未从授粉树上检测出黄龙病菌。说明花粉传播黄龙病缺乏证据。

In order to evaluate the possibility of pollen transmission of Citrus Huanglongbing(HLB),pollen was collected from Candidatus Liberibacter asiaticus(Las)-infected Citrus maxima(Burm.)Merr.cv.Shatian T.T.Yu.And the pollen was pollinated to the flowers of six healthy Citrus unshiu Marc trees.The symptoms of the pollinated trees were surveyed 30 days,60 days,90 days and 180 days after pollination,respectively.Meanwhile,detection of Las using real-time fluorescence quantitative PCR method in the pollinated trees was also performed.Results showed that all the trees pollinated with Las-infected pollen grew well even 180 days after pollination,and no Las was detected from any of the pollinated trees.This study indicates that there is lack of evidence that HLB could be disseminated through pollination pathway.