天然牛磺酸对急性抑郁模型小鼠的干预作用

Intervention Effect of Natural Taurine on Acute Depression Model Mice

以天然牛磺酸为试验材料,通过建立2种急性抑郁动物模型,研究牛磺酸对抑郁模型动物的干预效果。以ICR小鼠为实验动物,设牛磺酸低(0.5 g/kg bw)、中(1 g/kg bw)、高(2 g/kg bw)剂量组,同时设溶剂对照组、模型对照和阳性(氟西汀)对照组。通过建立行为绝望动物模型,探讨天然牛磺酸对行为绝望小鼠的干预效果;通过行为学、血清和脑组织中相关生化指标,分析牛磺酸对利血平诱导的急性抑郁的干预机制。结果表明:牛磺酸中、高剂量组可显著改善行为绝望小鼠的抑郁样行为,并缓解利血平诱导的小鼠眼睑下垂,同时升高抑郁小鼠脑组织五羟色胺、去甲肾上腺素和多巴胺水平,降低单胺氧化酶活性,提高脑源性神经营养因子含量,降低促肾上腺皮质激素释放因子、促肾上腺皮质激素、皮质醇水平,改善相关生化指标。研究表明:牛磺酸对行为绝望模型和利血平诱导抑郁模型均具有干预抑郁的作用,作用机制可能与增加单胺类神经递质,参与调节肾上腺功能的紊乱有关。

Using natural taurine as experimental material,the intervention effect of taurine on two acute depression animal models by establishing two acute depression animal models.ICR mice were used as experimental animals.Low taurine(0.5 g/kg bw),medium taurine(1 g/kg bw)and high taurine(2 g/kg bw)dose groups were set.At the same time,solvent control group,model control group and positive(fluoxetine)control group were set.The intervention effect of natural taurine on behavioral despair mice was investigated by establishing behavioral despair animal model.The intervention mechanism of taurine on reserpine induced acute depression was analyzed through behavioral,biochemical indexes in serum and brain tissue.The results showed that taurine medium and high dose groups could significantly improve the depression-like behavior of behavioral despair mice.And ease of the mice induced by reserpine drooping eyelids,and higher depression in mice brain serotonin,norepinephrine and dopamine levels,reduce the monoamine oxidase activity,higher content of brain derived neurotrophic factor,reduce corticotrophin releasing factor,adrenocorticotropic hormone,cortisol levels,improve related biochemical indicators.It is showed that taurine can interfere with depression in both behavioral depression model and reserpine induced depression model,and the mechanism may be related to the increase of monoamine neurotransmitters and the regulation of adrenal dysfunction.