喷雾冷冻干燥技术制备乳双歧杆菌Probio-M8微胶囊制剂

Preparation of Bifidobacterium lactis Probio-M8 Microcapsule by Spray-Freeze-Drying Technology

目的:乳双歧杆菌Probio-M8是一株具有优良益生特性的有益菌,本试验以喷雾冷冻干燥工艺为基础,考察其对乳双歧杆菌M8微胶囊品质的影响,并分析干燥后微胶囊的特性。方法:采用单因素及Box-Behnken响应面试验优化最佳工艺条件,制备微胶囊。用扫描电镜观察微胶囊形态,用气相色谱法分析干燥前、后菌体细胞膜脂肪酸组分的变化,并模拟人体胃、肠液验证微胶囊的耐受性,探究其稳定性。结果:在干燥温度40℃,蠕动速度15 mL/min,喷嘴直径1.5 mm时,Probio-M8存活率最高为73.21%,微胶囊颗粒形态较为完整。干燥后不饱和脂肪酸含量显著提高10.53%(P<0.05),可维持细胞膜的流动性。在模拟人体胃、肠液耐受试验中,胶囊中的乳双歧杆菌Probio-M8平均存活率达81.99%,具有较好的胃、肠液耐受特性。结论:通过研究喷雾冷冻干燥法制备微胶囊的工艺,确定其最佳工艺条件以及对微胶囊品质的影响,为工业规模高质量和快速生产乳双歧杆菌Probio-M8微胶囊提供技术支持。

Objective:Bifidobacterium lactis Probio-M8(B.lactis Probio-M8)is a beneficial strain with excellent probiotic properties.This test is based on the spray-freeze-drying process.The aim is to investigate the effect of process conditions on the quality of B.lactis Probio-M8 microcapsules and its characteristics after drying.Methods:To prepare Bifidobacterium microcapsules,the single factor and Box-Behnken response surface tests were used to optimize the optimal process conditions.The microcapsule morphology was observed using scanning electron microscopy.Gas chromatographic analysis was used to analyze the changes in the fatty acid composition of the bacterial cell membrane before and after drying.The tolerance of microcapsules was verified by simulating human gastrointestinal fluid,and its stability was explored.Results:When the drying temperature was 40℃,the peristaltic speed was 15 mL/min,and the nozzle diameter was 1.5 mm,the survival rate was 73.21%,and the microcapsule particle form was relatively complete.After drying,the unsaturated fatty acid content increased significantly by 10.53%(P<0.05),maintaining the fluidity of the cell membrane.In the simulated human gastrointestinal fluid tolerance test,the average survival rate of B.lactis M8 in the capsule can reach 81.99%,which has good gastrointestinal fluid tolerance characteristics.Conclusion:By exploring the technology of spray-freeze-drying to prepare microcapsules,the preliminary determination of its optimal process conditions and its impact on the quality of microcapsules has opened up a new high-quality and rapid production of Bifidobacterium lactis Probio-M8 microcapsules on an industrial scale prospect.