剑麻EST-SSR在丝兰麻和中美麻中的通用性分析

Transferability Analysis of Sisal EST-SSR Markers in Yucca and Furcraea vent

采用聚丙烯酰胺凝胶电泳技术,对剑麻‘H.11648’中的100对SSR引物在丝兰麻和万年兰中的通用性进行分析,同时构建DNA指纹图谱。结果表明100对SSR引物,在龙舌兰属、丝兰麻属、中美麻属中分别有68对、52对和52对SSR引物扩增出目标产物条带,扩增产物所占比例分别为68%、52%和52%,多态性引物分别为18对、12对和9对,多态性引物所占比例分别为29.51%、23.08%和17.31%。检测位点数分别为76、44和40个,平均每对引物多态位点数分别为2.67、2.42和2.22个。引物C67656和C61418可将龙舌兰属的6份种质区分开来,引物C65059可将丝兰麻属的5份种质区分开,引物C24110可将中美麻属的4份种质区分开。以上研究结果表明,从‘H.11648’开发的SSR引物在龙舌兰及其近缘属中是可以通用的,筛选出的SSR引物可用于龙舌兰及其近缘属种质资源的鉴定和遗传多样性分析等研究。

In this study,polyacrylamide gel electrophoresis was used to analyze the generality of 100 pairs of SSR pri-mers from‘H.11648’in Agave and its related genus,and DNA fingerprinting was constructed.The results showed that 68,52 and 52 pairs of SSR primers could amplify the expected products in Agave,Yucca and Furcraea vent,respectively.The proportion of amplified products was 68%,52%and 52%.18,12 and 9 pairs of SSR primers showed polymorphic and the polymorphic percentage was 29.51%,23.08%and 17.31%.The total number of polymorphic loci was 76,44 and 40 with average number of polymorphic loci amplified by each primer was 2.67,2.42 and 2.22,respectively.Primers C67656 and C61418 could discriminate six germplasms in Agave,Primer C65059 could discriminate five germplasms in Yucca,Primer C24110 could discriminate four germplasms in Furcraea vent.The above results indicated that the SSR primer in‘H.11648’was transferable in Agave and its related genera,which laid a foundation for germplasm identification and genetic diversity analysis of Agave and its related genera.