摘要
Extracellular vesicles or exosomes are membrane encapsulated biological nanometric particles secreted virtually by all types of cells throughout the animal kingdom.Tey carry a cargo of active molecules to proximal and distal cells of the body as mechanism of physiological communication,to maintain natural homeostasis as well as pathological responses.Exosomes carry a tremendous potential for liquid biopsy and therapeutic applications.Tus,there is a global demand for simple and robust exosome isolation methods amenable to point-of-care diagnosis and quality control of therapeutic exosome manufacturing.Tis can be achieved by molecular profling of the exosomes for use with specifc sets of molecular-markers for diagnosis and quality control.Liquid biopsy is undoubtedly the most promising diagnosis process to advance“personalized medicine.”Currently,liquid biopsy is based on circulating cancer cells,cell free-DNA,or exosomes.Exosomes potentially provide promise for early-stage diagnostic possibility;in order to facilitate superior diagnosis and isolation of exosomes,a novel platform is developed to detect and capture them,based on localized surface plasmon resonance(LSPR)of gold nanoislands,through strong afnity between exosomes and peptide called Venceremin or Vn96.Physical modeling,based on the characteristics of the gold nanoislands and the bioentities involved in the sensing,is also developed to determine the detection capability of the platform,which is optimized experimentally at each stage.Preliminary results and modeling present a relationship between the plasmonic shif and the concentration of exosomes and,essentially,indicate possibilities for label-free early diagnosis.
基金
The authors acknowledge the support of M.Packirisamy from Natural Sciences and Engineering Research Council of Canada(NSERC)and Concordia Research Chair and New Brunswick Innovation Foundation(NBIF).
