急性脑梗死患者外周血miR-497-5p表达的变化及临床意义

Changes and clinical significance of miR⁃497⁃5p expression in peripheral blood of patients with acute cerebral infarction

目的研究急性脑梗死患者外周血miR-497-5p表达与炎症因子、氧化应激产物释放及90 d预后的关系。方法选择2018年4月至2020年4月期间本院收治的132例急性脑梗死患者作为急性脑梗死组、体检的106例健康志愿者作为对照组,检测外周血miR-497-5p的表达水平及血清炎症因子IL-1β、IL-6、TNF-α、氧化应激产物MDA、AOPP、8-OHdG的含量,随访急性脑梗死患者90 d预后。结果急性脑梗死组患者外周血miR-497-5p的表达水平高于对照组,差异有统计学意义(P<0.05);急性脑梗死组中miR-497-5p高表达患者的血清IL-1β、IL-6、TNF-α、MDA、AOPP、8-OHdG的含量高于miR-497-5p低表达患者,差异有统计学意义(P<0.05);急性脑梗死组中90d预后良好患者外周血中miR-497-5p的表达水平低于预后不良患者,差异有统计学意义(P<0.05)。且外周血miR-497-5p的表达水平对急性脑梗死患者的预后具有预测价值。结论急性脑梗死患者外周血miR-497-5p的异常高表达与炎症因子、氧化应激产物释放增多及90d预后不良有关。

Objective To study the relationship between the expression of miR⁃497⁃5p in peripher⁃al blood of patients with acute cerebral infarction and the release of inflammatory factors,oxidative stress prod⁃ucts and the prognosis of 90 days.Methods 132 cases of patients with acute cerebral infarction in our hospital from April 2018 to April 2020 were selected as the acute cerebral infarction group and 106 cases of healthy vol⁃unteers were selected as the control group.The expression level of miR⁃497⁃5p in peripheral blood and the con⁃tents of serum inflammatory factors IL⁃1β,IL⁃6,TNF⁃α,oxidative stress products MDA,AOPP and 8⁃OHdG were detected.The prognosis of patients with acute cerebral infarction was followed up for 90 days.Results The expression level of miR⁃497⁃5p in peripheral blood of patients in the acute cerebral infarction group was higher than that in the control group,the difference was statistically significant(P<0.05).the levels of serum IL⁃1β,IL⁃6,TNF⁃α,MDA,AOPP and 8⁃OHdG in patients with high miR⁃497⁃5p expression in acute cere⁃bral infarction group were higher than those in patients with low miR⁃497⁃5p expression,the difference was sta⁃tistically significant(P<0.05).In the acute cerebral infarction group,the expression level of miR⁃497⁃5p in the peripheral blood of patients with good prognosis at the day of 90 was lower than that of patients with poor prog⁃nosis,and the difference was statistically significant(P<0.05).The expression level of miR⁃497⁃5p in peripher⁃al blood had predictive value for the prognosis of patients with acute cerebral infarction.Conclusion The ab⁃normally high expression of miR⁃497⁃5p in peripheral blood of patients with acute cerebral infarction is related to the increased release of inflammatory factors,oxidative stress products and poor prognosisat 90 days.