全集成离心式微流控农残检测芯片研制

Design of fully-integrated centrifugal microfluidic chip for rapid detection of pesticide residues

基于微流控技术和酶抑制法的原理,研制了一种集成液体生化试剂的离心式农残速测芯片。采用透明聚乙烯膜制备微型液囊储存液体试剂,与预先固化反应试剂构成全集成微流控圆盘芯片,用于农药残留的快速检测,实现了从样品提取、酶抑制反应到显色反应及检测的全集成和自动化操作。结果表明,在芯片内部集成农残提取单元,可以省去常规方法所必需的前处理设备,提高检测效率,降低检测人员工作强度,同时检测结果的准确性可以满足大批量样品的农药残留筛查需求。

Based on the principles of microfluidic technology and enzyme inhibition,a centrifugal chip integrated with liquid biochemical reagents was developed for the rapid measurement of pesticide residues.The liquid reagents have been stored in a microcapsule made of transparent polyethylene(PE)film,combined with the pre-cured reagents that form a fully integrated microfluidic disk chip.The detection procedure(sample extraction,inhibition of enzyme reactions,color response)could be performed automatically in the chip using a portable device.The results showed that the integration of extraction units for pesticide residues inside the chip could eliminate the pretreatment equipment of the conventional method,improved the detection efficiency,and reduced the work intensity of the inspectors.At the same time,the accuracy of the results could meet the pesticide residue screening of batch samples.