基于网络药理学探讨补肺活血胶囊用于重症肺炎患者康复期治疗的关键成分和作用机制

Discovery of the active ingredients and the mechanism of action for the effects of Bufei Huoxue Capsule on pneumonia recovery based on network pharmacology methods

运用网络药理学技术,探究了补肺活血胶囊用于重症肺炎患者康复期治疗的关键成分及作用机制,为补肺活血胶囊促进肺炎患者康复提供依据。使用TCMSP和CTD数据库检索补肺活血胶囊中化合物的作用靶点,再与SwissTargetPrediction数据库预测的潜在作用靶点进行整合,得到补肺活血胶囊成分的全部潜在靶基因。运用String数据库进行蛋白互作分析(PPI),筛选出补肺活血胶囊的核心靶点,找到对应的化合物,并构建药材-关键成分-核心靶点网络。采用David数据库对核心靶点进行研究,明确补肺活血胶囊的作用机制。共搜集到补肺活血胶囊作用的靶点1 102个,筛选得到核心靶点215个,主要涉及AKT1、MAPK3、VEGFA、CASP3和EGFR等。KEGG通路富集得到TNF、T cell receptor、Rap1、PI3K-Akt、HIF-1和FoxO等相关信号通路,为补肺活血胶囊促进肺炎患者康复的潜在靶标通路。补肺活血胶囊能通过多靶点多通路抑制机体炎症,缓解患者的肺纤维化程度,加速恢复期患者的康复,有效地促进肺炎患者的康复。

The study aimed to explore the mechanism of action of Bufei Huoxue Capsule(BFHXC)for treatment of pneumonia recovery through network pharmacology. The constituents of BFHXC were collected by preliminary studies. The targets of constituents were collected by TCMSP and Toxicogenomics Database(CTD)as well as by prediction of SwissTargetPrediction based on structural similarity. Proteinprotein interaction(PPI)data was constructed by using String website for screening key targets. The herb-active ingredient-key target network was constructed by using Cytoscape software,followed by topology analysis. The DAVID database was used to perform GO enrichment analysis and KEGG pathway analysis on the key targets for predicting the mechanism of action of BFHXC. A total of 1 102 targets were collected for BFHXC,and 215 key targets were screened,such as AKT1,MAPK3,VEGFA,CASP3,EGFR and so on. The results of pathway enrichment indicated that TNF signaling pathway,T cell receptor signaling pathway,Rap1 signaling pathway,PI3 K/Akt signaling pathway,HIF-1 signaling pathway and FoxO signaling pathway were the key pathways,which were involved in the treatment of pneumonia recovery. BFHXC may exhibit therapeutic effects on pneumonia recovery probably through inhibiting inflammation and relieving pulmonary fibrosis based on multi-target and multi-pathway.