基于网络药理学研究人参防治创伤性应激障碍药效成分及作用靶点

Study on active components and targets of Ginseng inPrevention and treatment of PTSD based on Network Pharmacology

目的通过网络药理学研究方法获得人参防治创伤性应激障碍(PTSD)药效成分及作用靶点。方法利用TCMSP研究平台获取人参具有药用价值的有效成分及作用靶点信息,检索GeneCard和OMIM数据库获取与PTSD发病密切相关的基因信息,将两者进行匹配获得人参防治PTSD的药用成分和靶点基因。应用R和Perl两种计算机语言绘制药物-疾病-靶点网络图,并对靶点基因进行PPI网络分析、基因本体论(GO)和信号转导通路(KEGG)富集分析。结果根据中药药理学通行研究方法设定药效成分筛选参数,获得11种人参高价值药用成分。11种药用成分与PTSD疾病相关的16个靶基因具有药靶作用关系。经过PPI、GO和KEGG分析发现靶基因主要富集在神经递质、突触可塑性、离子通道等生理功能上。结论人参具有防治PTSD的药理作用,可能通过调控单胺类神经递质代谢及受体活性途径发挥作用。

Aim To obtain the active components and targets of ginseng in the prevention and treatment of traumatic stress disorder(PTSD)through the method of network pharmacology.Methods The active components and target information of ginseng with medicinal value were obtained by TCMSP research platform,and the gene information closely related to the pathogenesis of PTSD was obtained by searching GeneCard and OMIM database.The two were matched to obtain the medicinal components and target genes of ginseng in the prevention and treatment of PTSD.The drug-disease-target network diagram was drawn by R and Perl computer languages,and the target genes were analyzed by PPI network analysis,gene ontology(GO)and signal transduction pathway(KEGG)enrichment analysis.Results According to the general pharmacological research methods of traditional Chinese medicine,the screening parameters of active components were set,and nine kinds of high value medicinal ingredients of Panax ginseng were obtained.There was a drug-target relationship between the nine medicinal components and sixteen target genes related to PTSD disease.Through PPI,GO and KEGG analysis,it was found that the target genes were mainly enriched in physiological functions such as neurotransmitters,synaptic plasticity,ion channels and so on.Conclusions Ginseng has the pharmacological effect of preventing and treating PTSD,which may play a role in regulating the metabolism and receptor activity of monoamine neurotransmitters.