11个玉米DOF基因对逆境胁迫的表达模式分析

Identification and Expression Pattern Analysis of 11 ZmDOFs under Abiotic Stress in Maize

研究玉米DOF基因在玉米不同组织和非生物胁迫条件下的响应表达模式,为进一步阐明玉米DOF基因的生物学功能提供有价值的信息。利用qRT-PCR技术探测11个玉米DOF基因在‘郑58’自交系不同组织中以及在200 mmol/L NaCl溶液、20%PEG6000溶液和铵态氮、硝态氮缺乏胁迫等条件下的表达模式。进化树分析结果表明,这11个ZmDOF基因聚为一个家族,可进一步划归为4组。表达分析结果表明,有6个ZmDOF基因具有组织特异性表达模式,集中在雄花中表达,表明这6个ZmDOF基因在雄花生长发育进程中发挥作用。ZmDOF22和ZmDOF40没有检测到表达,可能是表达量太低或者特异时空表达模式。在模拟盐和干旱胁迫下,多数ZmDOF基因表达上调,其中分别有2个和6个基因的表达上调至少5倍。在铵态氮或硝态氮胁迫处理下,分别有3个和5个基因的表达量上调至少5倍,而ZmDOF2和ZmDOF18/ZmDOF39的表达量明显受到抑制。结果预示这11个ZmDOF基因可能广泛参与玉米生长发育以及抵御各种逆境胁迫的响应途径。

The response expression pattern of the ZmDOF genes was studied in different tissues of maize under condition of abiotic stress,which canprovide valuble information to further clarify the biological function of DOF genes.The quantitative RT-PCR(qRT-PCR)method was used to detect the expression profiling of 11 ZmDOF genes in six different tissues of maize inbred-line‘Zheng 58’under conditons of 200 mmol/L NaCl,20%PEG6000,ammonium nitrogen and nitrate nitrogen starvation stresses.The phylogenetic analysis showed that these genes belonged to one family,which could be further subdivided into four groups.The tissue expression analysis showed that 6 ZmDOF genes had tissue specific expression patterns,especially high expression in tassels,indicating the importance of ZmDOF genes during tassel growth and development in maize.ZmDOF22 and ZmDOF40 were not detected probably due to very lower expression level or specific spatiotemporal expression pattern.The results indicated that most ZmDOFs were obviously induced under salt or osmotic stresses and 2 or 6 ZmDOFs were up-regulated by 5-fold,respectively.Under ammonium nitrogen or nitrate nitrogen starvation stresses,the expression level of 3 or 5 ZmDOF genes were upregulated by 5-fold,respectively,whereas ZmDOF2,ZmDOF18 and ZmDOF39 were obviously inhibited.So 11 ZmDOF genes are widely involved in maize growth and development or play important roles in stress response,this will provide valuable information for further elucidating the biological function of DOF gene in maize.