低温胁迫下冬油菜陇油7号根部代谢组学分析

Metabolomics profiling of Brassica campestris Longyou 7 roots under cold stress

为了解白菜型冬油菜陇油7号超强抗寒生理机制,采用液相色谱质谱(LC-MS)分析了低温胁迫条件下陇油7号根部代谢组学变化。KEGG富集分析发现,变化显著的代谢通路为:果糖和甘露糖代谢、淀粉和蔗糖代谢、花生四烯酸代谢、苯丙氨酸代谢、甘油磷脂代谢、半乳糖代谢、α-亚麻酸代谢、氨基酸生物合成、精氨酸和脯氨酸代谢以及半胱氨酸和蛋氨酸代谢。糖类、氨基酸、甘油磷酰胆碱、黄酮、6种磷脂(PG、PC、PE、PI、PS和PA)等均得到鉴定,其中糖(除鼠李糖外)、赖氨酸-缬氨酸、脯氨酸-色氨酸和黄酮在低温处理后差异倍数均大于1.2,磷脂中PC在低温胁迫时增加,PC(13∶0/0∶0)亚类差异倍数最高,为5.295,PE和PS在低温胁迫后下降,差异倍数0.528~0.740,PI、PA和PG类的变化因亚类而异。

The metabolomics changes of‘Longyou 7’roots under low temperature stress were analyzed by liquid chromatography mass spectrometry(LC-MS)to clarify the physiological mechanism of ultra strong cold resistance Brassica campestris variety‘Longyou 7’.KEGG enrichment analysis revealed that the metabolic pathways with significant changes were:fructose and mannose metabolism,starch and sucrose metabolism,arachidonic acid metabolism,phenylalanine metabolism,glycerophospholipid metabolism,galactose metabolism,α-linolenic acid metabolism,amino acid biosynthesis,arginine and proline metabolism,and cysteine and methionine metabolism.Sugars,amino acids,glycerophosphocholine,flavones and six kinds of phospholipids(PG,PC,PE,PI,PS and PA)were identified.Of which the fold change of sugar(except rhamnose),lysine-valine,proline-tryptophan and flavones were all over 1.20 under low temperature stress.The PC in phospholipid increased under cold stress,the fold change of the subclass PC(13∶0/0∶0)reached its highest point,which was 5.295.PE,and PS decreased under cold stress,the fold change was 0.528~0.740.Changes in PI,PA and PG were different for subclass.The results of this study provided reference for further research on the physiological characteristics on cold resistance of Brassica campestris.