马铃薯StSnRK2基因家族的鉴定与表达分析

Identification and Expression Profile of StSnRK2 Family Genes in Potato

研究利用最新报道(2020年)的杂合二倍体马铃薯‘RH89-039-16’基因组信息鉴定出23个马铃薯StSnRK2家族成员,分别被命名为StSnRK2.1~StSnRK2.23。通过生物信息学方法,分析了其基因结构、保守基序、理化性质、共线性关系,研究了其与水稻、玉米和拟南芥的SnRK2的系统进化关系,并利用转录组测序技术探究了家族成员在不同逆境胁迫下的基因表达规律。结果表明,马铃薯StSnRK2家族成员不均匀地分布在12条染色体上,家族成员蛋白长度为298~491 aa,分子量为32.6~55.4 kD,等电点4.51~9.47。系统进化树分析发现该基因家族可分为3个亚族,分别含有13、8和2个StSnRK2基因,在这些成员中共找到10个保守基序,其中保守基序1~9均包含于3个亚组中,而保守基序10则特异性分布于亚组Ⅰ。23个StSnRK2成员的表达模式表明,有15个基因在ABA胁迫24 h时表达量发生显著上调,证明这部分基因对马铃薯响应ABA胁迫具有重要的作用。这些结果为阐明StSnRK2基因家族的进化关系和进一步研究StSnRK2基因的功能特性提供了有价值的信息。

In the recent study(2020),23 StSnRK2 family members were identified from the reference genome of the newly reported hybrid diploid potato'RH89-039-16'.All the members were named from StSnRK2.1 to StSnRK2.23.The gene structure,conserved motifs,collinearity and physiochemical properties were predicted through bioinformatics methods.Furthermore,the phylogenetic relationships of StSnRK2 family members was analyzed among rice,maize and Arabidopsis,and the gene expression patterns of StSnRK2 family members was explored under different stress conditions using transcriptome sequencing technology.StSnRK2 members were unevenly assigned into 12 chromosomes,and there members contained from 298 to 491 amino acids.Their relative molecular weight varied from 32.6 to 55.4 kD,with isoelectric points ranging from 4.51 to 9.47.Phylogenetic analyses showed that 23 StSnRK2 proteins were divided into three subfamilies,including 13,8 and 2 genes,respectively.A total of 10 conserved motifs were found in these members,among which conserved motifs from 1 to 9 were all contained in three subgroups,while the 10 conserved motifs were specifically distributed in subgroup I.The expression patterns of 23 StSnRK2 members showed that 15 genes were significantly up-regulated under ABA stress for 24 h,indicating that these genes play a vital role in potato response to ABA stress.These findings provide useful information to elucidate StSnRK2 gene family's evolutionary relationship and to further analyze the functional characteristics of StSnRK2 gene.