以酸枣仁、茯苓为主的复方固体饮料改善睡眠的作用研究

Study on the effect of a compound solid beverage mainly composed of semen Ziziphi spinosae and Poriacocos on improving sleep

目的研究以酸枣仁、茯苓为主的复方固体饮料改善睡眠的作用。方法选取120只SPF级健康雄性Balb/c小鼠,随机分组,每组10只,每天经口灌胃给予阴性对照组(生理盐水)、固体饮料低、中、高3个剂量(6.5、13、19.5 g/kg),连续灌胃30 d后,进行直接睡眠实验、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下剂量催眠实验、巴比妥钠睡眠潜伏期实验;使用酶联免疫吸附实验法(enzyme linked immunosorbent assay,ELISA)检测脑组织中5-羟色胺(5-hydroxytryptamine,5-HT)、多巴胺(dopamine,DA)、γ-氨基丁酸(γ-aminobutyric acid,GABA)的含量。结果复方固体饮料无直接睡眠作用。与阴性对照组相比,复方固体饮料低、中、高剂量小鼠睡眠时间分别延长了42.63%、54.09%、53.55%,均具有统计学差异(P<0.001);各剂量组小鼠30min内小鼠阈下剂量催眠实验均为阳性,小鼠的睡眠发生率增加,差异具有统计学意义(P<0.001);各剂量组小鼠巴比妥钠睡眠潜伏期时间显著缩短(P<0.001),差异具有统计学意义;复方固体饮料低剂量组能显著提高小鼠脑内5-HT和GABA含量(P<0.01);复方固体饮料中、高剂量组能使小鼠脑内5-HT、DA和GABA酸含量显著升高(P<0.001),差异具有统计学意义。结论复方固体饮料具有明显的促睡眠作用,与戊巴比妥钠的协同作用较强,可能与脑组织中GABA、5-HT、DA水平的提高有关。

Objective To study the effect of a compound solid drink with Ziziphi spinosae and Poriacocos as the main ingredient on improving sleep.Methods A total of 120 SPF grade healthy male Balb/c mice were randomly divided into 10 mice in each group.The negative control group normal saline and different doses of solid beverage were given 3 doses(6.5,13,19.5 g/kg)every day.Then direct sleep test,prolonged sodium pentobarbital sleep time experiment,hypnosis experiment under subthreshold dose of sodium pentobarbital and barbital sodium sleep latency test were conducted.The content of 5-hydroxytryptamine(5-HT),dopamine(DA)andγ-aminobutyric acid(GABA)in brain tissues were detected by enzyme linked immunosorbent assay(ELISA).Results Solid beverage had no direct sleep effect.Compared with the negative control group,the sleep time of mice with low,medium and high doses of solid beverage was extended by 42.63%,54.09%and 53.55%,respectively,showing statistical differences(P<0.001).The subthreshold dose hypnosis test of mice in each dose group within 30 minutes was positive,the incidence of sleep in mice increased,and the difference was statistically significant(P<0.001).The sleep latency time of barbital sodium in each dose group was significantly shortened,and the difference was statistically significant(P<0.001).The content of 5-HT and GABA in the brain of mice was significantly increased(P<0.01).The content of 5-HT,DA and GABA acid in the brain of mice was significantly increased(P<0.001)in solid beverage and high dose(P<0.001),and the difference was statistically significant.Conclusion Solid beverage have obvious sleep promoting effect,and the synergistic effect with pentobarbital sodium is stronger,which may be related to the increase of GABA,5-HT and DA levels in brain tissue.