高蛋白保健品促进髋关节骨折愈合研究

Promotion of hip fracture healing by high protein health care products

目的建立小鼠髋关节骨折模型,探讨高蛋白保健品对骨折愈合的影响。方法将小鼠分为空白对照组和实验组,对照组以等体积的生理盐水灌胃,实验组分别以低、中、高3个剂量组0.15、0.75、1.5 g/kg·d,每天1次给小鼠灌胃28d,采用X光检查法、组织病理切片法、血清中胰岛素样生长因子-1法(insulin-like growth factor-1, IGF-1)和碱性磷酸酶(alkaline phosphatase, ALP)含量测定法等,观察不同蛋白含量对骨折模型小鼠的影响。结果与对照组比,高蛋白含量实验组的小鼠骨折愈合明显加快。X光检查结果显示,髋关节骨折模型成模率整体达到89%,表明建模稳定;苏木精-尹红染色实验结果证明, 1.5 g/kg·d实验组骨痂组织状态明显,骨性连接密度浓稠,骨髓腔充满大量骨髓细胞;实验小鼠组血清中IGF-1和ALP含量较对照组更高,更利于骨的形成。生物力学实验结果证明高蛋白剂量组骨痂机械强度更高、抗压能力更强。结论通过对骨折小鼠饲喂不同含量的蛋白质保健品,发现高蛋白对其术后愈合具有明显的改善效果。

Objective To establish a mouse model of hip fracture, and explore the effect of high protein health care products on fracture healing. Methods The mice were divided into blank control group and experimental groups. The control group was gavaged with the same volume of normal saline. The experimental groupsweregavaged with low, medium and high dose groups of 0.15, 0.75 and 1.5 g/kg·d once a day for 28 d. Then X-ray examination, histopathological section, serum insulin-like growth factor-1(IGF-1) and alkaline phosphatase were used. The effects of different protein contents on fracture model mice were observed. Results Compared with the control group, the fracture healing of high protein content experimental groups were significantly faster. The results of X-ray examination showed that the overall modeling rate of hip fracture model reached 89%, which indicated that the modeling was stable. The results of hematoxylin-eosin staining showed that the callus tissue of 1.5 g/kg·d experimental group was in obvious state, the density of bone connection was thick, and the bone marrow cavity was filled with a large number of bone marrow cells. The serum IGF-1 and ALP contents of experimental groups were higher than those of control group, which were more conducive to bone formation. The results of biomechanical test showed that the mechanical strength and compressive strength of callus were higher in high protein dose group. Conclusion Through feeding different content of protein health care products to fracture mice, it is found that high protein can significantly improve the postoperative healing.