含乳特殊医学用途配方食品中沙门氏菌检测即用型质控样品研制

Development of Ready-to-Use Quality Control Samples for the Detection of Salmonella in Formula Foods for Special Medical Purposes Containing Milk

为评估即用型沙门氏菌质控样品在基质辅助激光解析电离飞行时间质谱技术鉴定含乳特殊医学用途配方食品中沙门氏菌中的应用价值,通过验证即用型沙门氏菌质控样品的均匀性、稳定性,对-20℃贮藏0、14、28 d沙门氏菌质控样品进行质谱鉴定,最后用VITEK全自动微生物分析系统进行生化鉴定。结果表明:即用型沙门氏菌质控样品稳定性良好、均匀性符合要求,所得质谱图相近,VITEK生化鉴定结果为沙门氏菌;即用型沙门氏菌质控菌株可以作为含乳特殊医学用途配方食品中沙门氏菌检测质控样品使用。

In order to evaluate the application value of ready-to-use Salmonella quality control samples in matrix assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS)identification of Salmonella in milkcontaining formula foods for special medical purposes,this experiment evaluated the uniformity and stability of the quality control samples.After being stored at−20℃for 0,14 and 28 days,these samples were identified by mass spectrometry and the VITEK automatic microbial analysis system.The results showed that the quality control samples had good stability and uniformity,and their mass spectra were similar to each other.The VITEK system confirmed their identity as Salmonella.In conclusion,the ready-to-use Salmonella quality control strains can be used for the detection of Salmonella in milk-containing formula foods for special medical purposes.