白术-苍术药对干预溃疡性结肠炎作用机制的网络药理学研究

Network pharmacological study on the mechanism of action of Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma couplet medicines on the intervention of ulcerative colitis

目的基于网络药理学分析白术-苍术药对干预溃疡性结肠炎(UC)的作用机制。方法通过中药系统药理学数据库和分析平台与中药分子机制的生物信息学分析工具分别筛选白术、苍术活性成分和靶基因;使用人类基因数据库GeneCards获得UC相关基因;使用Cytoscape软件构建“药物-活性成分-靶点-疾病”网络;通过String数据库分别构建苍术-白术药对治病靶点蛋白质-蛋白质相互作用网络图;使用R语言软件对关键靶基因进行GO功能富集和KEGG通路富集分析。结果共筛选出16个药物有效活性成分;共检索出4209个UC相关基因;药对与疾病相关的共同靶点共筛选出31个;核心基因主要有E1A结合蛋白P300、肿瘤坏死因子-α、丝裂原活化蛋白激酶1(MAPK1)等;信号通路主要有磷酸肌醇-3激酶/蛋白激酶B、核因子-κB、人类T细胞白血病病毒1型、MAPK、缺氧诱导因子-1等信号通路。结论通过构建“药物-活性成分-靶点-疾病”网络,从多成分、多靶点、多通路阐释白术-苍术药对干预UC的作用机制,为后续深入研究提供依据和参考。

Objective To analyze the mechanism of action of Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma couplet medicines on the intervention of ulcerative colitis(UC)based on network pharmacology.Methods The active components and target genes of Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma were screened by traditional Chinese medicine systems pharmacology database and analysis platform and bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine.UC-related genes were obtained using GeneCards,a human gene database.Cytoscape software was used to build the“drug-active component-target-disease”network.Proteinprotein interaction network diagrams of Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma couplet medicines on disease targets were constructed by String database.GO functional enrichment and KEGG pathway enrichment analysis of key target genes were performed using R language software.Results A total of 16 active ingredients were screened out.A total of 4209 UC-related genes were retrieved.A total of 31 common targets of couplet medicines and disease-related were screened out.The main core genes included E1A binding protein P300,tumor necrosis factor-α,mitogen-activated protein kinase 1(MAPK1),etc.The main signaling pathways included phosphoinositol-3 kinase/protein kinase B,nuclear factor-κB,human T-cell leukemia virus type 1,MAPK,hypoxia-inducible factor-1,etc.Conclusion By building a“drug-active component-target-disease”network,from multiple components,multiple targets,multiple pathways to interpret mechanism of action of Atractylodis Macrocephalae Rhizoma and Atractylodis Rhizoma couplet medicines to intervene in the UC,provide the basis for subsequent research and reference.