摘要
目的建立血液中氟阿普唑仑的高效液相色谱-串联质谱(HPLC-MS/MS)检验方法。方法采用快速溶剂萃取(ASE)对血液检材中氟阿普唑仑进行提取,供高效液相-串联质谱检验。结果在血液中氟阿普唑仑0.1~100ng/mL范围内,峰面积与浓度呈良好的线性关系,最低检出限为0.05ng/mL,定量限为0.1ng/mL,回收率为75.4%~95.3%。结论该方法萃取速度快、操作简单、检出限低、回收率高和重现性好,适用于血液中氟阿普唑仑的检验。
Objective To establish an LC-MS/MS method for determination of flualprazolam in blood.Methods Automatic rapid solvent extraction(ASE)was adopted to extract flualprazolam in blood.The extracted chemical was subjected to analysis of LC-MS/MS.Results Good linear relationship was present with peak area among 0.1~100ng/mL of flualprazolam in blood,with the involving limit of detection(LOD)being 0.05ng/mL,limit of quantification as 0.1ng/mL,and recoveries within the range of 75.4%~95.3%.Conclusion This method is of fast extraction speed,simple operation,low detection limit,high recovery rate and good reproducibility,therefore suitable for determination of flualprazolam in blood.
作者
蔡玉刚
吴永富
邹波
王文
张莲
常靖
CAI Yugang;WU Yongfu;ZOU Bo;WANG Wen;ZHANG Lian;CHANG Jing(Luzhou Institute of Forensic Science,Luzhou 646000,Sichuan,China;Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China;Sichuan Police College,Luzhou 646000,Sichuan,China)
出处
《刑事技术》
2021年第4期374-377,共4页
Forensic Science and Technology
基金
公安部现场物证溯源技术国家工程实验室开放性课题(2018NELKFKT 13)。
