血浆miR-223和Fox03a水平与冠心病及其传统危险因素之间的相关性研究

Relationship between plasma miR-223 and Fox03a levels with coronary heart disease and its traditional risk factors

目的探讨血浆miR-223和FoxO3a水平与冠心病及传统危险因素之间的相关性。方法纳入2019年1月至2019年12月在新疆医科大学第一附属医院住院的冠心病患者126例(冠心病组)和非冠心病患者111例(对照组),两组平均年龄为(66.38±12.12)岁和(49.35±9.60)岁。通过酶联免疫分析法,检测所有研究对象血浆miR-223、FoxO3a的水平,分析miR-223、FoxO3a的水平与冠心病、年龄、血脂、血糖、血常规等指标的关系。结果两组在年龄、合并高血压、HDL-C、空腹血糖、血清胱抑素C、白细胞计数、中性粒细胞计数、单核细胞计数、血小板平均分布宽度差异具有统计学意义(t=6.954、12.014、2.197、2.996、3.021、2.300、2.577、2.111、3.955,P=0.000、0.001、0.031、0.004、0.030、0.024、0.012、0.038、0.000)。冠心病组miR-223[(0.415±0.065)比(0.309±0.057)]、FoxO3a[(0.402±0.058)比(0.306±0.059)]水平明显高于对照组,差异具有统计学意义(P<0.05)。相关分析研究中,miR-223和FoxO3a水平间呈正相关(r=0.485,P=0.000)。控制年龄变量后,miR-223和FoxO3a水平与冠心病之间仍呈正相关(r=0.388,P=0.000;r=0.506,P=0.000)。通过多重线形回归分析结果显示,冠心病组miR-223和FoxO3a水平的表达量更高,呈正相关。结论冠心病患者中miR-223和FoxO3a水平同时明显升高,呈高表达状态,可能成为冠心病新型生物学标记物。

Objective To investigate the correlation between plasma levels of miR-223 and Fox03a and coronary heart disease as well as its traditional risk factors.Methods From January 2019 to December 2019,126 patients with coronary heart disease and 111 patients without coronary heart disease were enrolled in the First Affiliated Hospital of Xinjiang Medical University,with an average age of(66.38±12.12)vs(49.35±9.60)years.The plasma levels of miR-223 and FoxO3 a were detected by enzyme-linked immunosorbent assay(ELISA).The relationship between the levels of miR-223 and FoxO3 a and coronary heart disease,age,blood lipid,blood glucose and blood routine were analyzed.Results There were significant differences in age,hypertension,HDL-C,fasting blood glucose,serum cystatin C,white blood cell count,neutrophil count,monocyte count and mean platelet distribution width between the two groups(t=6.954,12.014,2.197,2.996,3.021,2.300,2.577,2.111,3.955,P=0.000,0.001,0.031,0.004,0.030,0.024,0.0,respectively 012、0.038、0.000).The levels of miR-223[(0.415±0.065)vs.(0.309±0.057)]and Fox03a[(0.402±0.058)vs.(0.306±0.059)]in patients with coronary heart disease were significantly higher than those in patients without coronary heart disease(P<0.05).Multiple linear regression analysis showed that coronary heart disease was positively correlated with miR-223 and Fox03a levels and miR-223 and Fox03a levels were also positively correlated.Conclusion The levels of miR-223 and Fox03a in patients with coronary heart disease are significantly increased at the same time,showing a higher expression state,which may become a new biological marker of coronary heart disease.