未成熟卵母细胞体外成熟前后玻璃化冷冻的比较

Comparison on vitrification of immature oocytes before and after in vitro maturation

目的比较人未成熟卵母细胞体外成熟(in vitro maturation,IVM)前后玻璃化冷冻对卵母细胞的影响。方法318枚人生发泡期(germinal vesicle,GV)卵母细胞来自100名不孕症患者,将患者随机分为A、B两组,每组50人。A组GV期卵母细胞玻璃化冷冻后解冻复苏行IVM;B组GV期卵母细胞行IVM后玻璃化冷冻再解冻复苏,比较两组卵母细胞的解冻复苏率、成熟率及24小时纺锤体出现率。结果两组卵母细胞行IVM培养24小时及48小时成熟率比较均有统计学意义(P<0.05),卵母细胞解冻复苏率、IVM培养24小时纺锤体形成率比较无统计学意义(P>0.05)。结论未成熟卵母细胞IVM后再行玻璃化冷冻保存效果更佳。

Objective To compare the effect of vitrification of human immature oocytes before and after in vitro maturation(IVM).Methods A total of 318 human germinal vesicle(GV)human oocytes were collected from 100 infertile patients,and the patients were randomly divided into group A and group B,with 50 cases in each group.The oocytes in the GV stage of the group A were vitrified and thawed before IVM,and the oocytes in the GV stage of the group B were vitrified and thawed after IVM.And then,the thawing recovery rate,maturation rate and 24-hour spindle formation rate of oocytes in the two groups were compared.Results The maturation rates of oocytes cultured in IVM at 24 h and 48 h were statistically different between the group A and the group B(P<0.05),while there was no statistically significant difference in the thawing recovery rate of oocytes and the spindle formation rate after 24 h IVM culture between the two groups(P>0.05).Conclusion Vitrification of immature oocytes after IVM is more effective.