骨髓间充质干细胞延缓单侧输尿管梗阻模型肾脏纤维化的研究

The study on bone marrow-derived mesenchymal stem cells in alleviating tubulointerstitial injury in unilateral ureteral obstruction model

目的探讨骨髓间充质干细胞(MSCs)减轻单侧输尿管梗阻(UUO)模型肾脏损伤的效果及相关的机制。方法通过体外培养的方法制备MSCs,并将MSCs通过尾静脉注射的方法注入到UUO模型鼠体内,在造模的第14天检测不同组肾脏病理的改变、管周毛细血管、细胞凋亡及β-catenin表达情况来观察MSCs是否可以减轻肾脏损伤并探讨相关机制。结果将培养的MSCs通过尾静脉注射的方法注入到UUO模型BALB/C小鼠体内,发现在MSCs组肾脏病理纤维化的程度较UUO组明显减轻(HE:1.60±0.35比3.34±0.23;MASSON:21.32±7.54比51.08±4.45),同时MSCs注入可以进一步减少管周毛细血管的丢失(13.56±4.65比60.16±10.24)、抑制细胞的凋亡(14.32±3.54比28.16±6.21)及β-catenin表达(29.33±6.45比39.51±8.42)。结论MSCs治疗对慢性肾脏纤维化起到保护作用,作用机制与减少管周毛细血管丢失、抑制细胞凋亡及β-catenin的表达有关。

Objective To investigate the effect and mechanism of bone marrow-derived mesenchymal stem cells(MSCs)in alleviating renal injury in unilateral ureteral obstruction(UUO)model.Methods MSCs were cultured,and then transplanted into a UUO model through the tail vein.Histology,cell apoptosis,peritubular capillary(PTC)loss andβ-catenin expression were examined on the fourteen day after surgery.Results Renal interstitial fibrosis in the MSCs group was significantly attenuated compared with that in the UUO group(HE:1.60±0.35 vs.3.34±0.23;MASSON:21.32±7.54 vs.51.08±4.45).Moreover,MSCs treatment inhibited the loss of peritubular capillaries(PTC)(13.56±4.65 vs.60.16±10.24),cell apoptosis(14.32±3.54 vs.28.16±6.21)andβ-catenin expression(29.33±6.45 vs.39.51±8.42).Conclusions MSCs infusion is a promising therapeutic strategy for promoting kidney repair in chronic renal fibrosis model.The mechanism maybe that it inhibits the loss of peritubular capillaries(PTC),cell apoptosis andβ-catenin expression.