高盐摄入对小鼠肠道GLP-1表达的影响

Effect of High-salt Intake on GLP-1 Expression of the Intestine.

目的探讨高盐饮食对小鼠血糖、血压、血脂以及肠道胰高血糖素样肽1(glucagon-like peptide-1,GLP-1)的影响。方法实验于2020年8~11月在武汉大学人民医院动物实验中心和中心实验室进行。8周龄C57BL/6雄性小鼠20只,依照数字表法随机分为对照组和高盐组,每组各10只。高盐组予含8%的NaCl高盐特制饲料喂养,对照组予普通饲料喂养,实验时间8周。在实验末测量小鼠尾动脉血压,实验结束后测定空腹血糖(fasting blood glucose,FBG)、血脂水平,免疫组化检测小鼠小肠GLP-1的表达。结果与对照组比较,高盐饮食喂养8周后,高盐组空腹血糖显著升高(P<0.05)。高盐组小鼠血清血脂水平明显高于对照组(P<0.05)。高盐组收缩压、舒张压水平高于对照组,差异有统计学意义(P<0.05),高盐组小肠GLP-1表达降低。结论高盐饮食可以影响小鼠糖脂代谢、血压,其原因可能与肠道GLP-1水平有关。

Objective To compare the expression of Glucagon-Like Peptide-1(GLP-1)in the small intestine between high-salt intake and control mice.Methods The experiment was conducted in the Central Laboratory of People's Hospital of Wuhan University from August 2020 to November 2020.Eight-week-old male C57B/L6mice with SPF grades were fed a standard or high-salt diet for 8 weeks(10 in each group).Blood pressure(BP)was measured on the seventh week.Blood was sampled for fasting blood glucose(FBG),serum total cholesterol(TC),triglyceride(TG),high density lipoprotein-cholesterol(HDL-ch)and low density lipoprotein-cholesterol(LDL-ch)assays.Small intestinal tissue were analyzed for GLP-1 expression by immunohistochemistry.Results The systolic and diastolic BP were higher in high-salt diet than standard diet(P<0.05)with significant difference.Plasma glucose,TC,TG level were lower in high-salt diet than control mice(P<0.05).Compared to the control group,the expression of GLP-1 in the small intestine were lower.Conclusion Changes in glucose,lipid and blood pressure after a high-salt diet appear related to the changes in the expression of GLP-1 in the small intestine.