Tiger17促进口腔黏膜成纤维细胞的增殖和迁移

Tiger 17 Promotes the Proliferation and Migration of Human Oral Mucosa Fibroblast Cells

目的观察在不同浓度Tiger17作用于人口腔黏膜成纤维细胞(hOMF)不同时间后对细胞增殖和迁移的影响。方法培养hOMF细胞,CCK8和划痕实验检测不同浓度Tiger17作用于hOMF细胞不同时间后细胞增殖和迁移的变化;Western Blot检测hOMF细胞表达TGF-β1、Erk1/2和p-Erk1/2蛋白的水平。结果100μg/mL和200μg/mL Tiger17对hOMF的增殖活性在48 h明显高于对照组(P<0.05);100μg/mL Tiger17对hOMF的迁移率在24 h和48 h均明显高于对照组(P<0.01);不同浓度的Tiger17作用于hOMF 24 h和48 h后,细胞表达TGF-β1、p-Erk1/2蛋白升高,差异有统计学意义(P<0.05)。结论Tiger17使hOMF细胞表达TGF-β1、pErk1/2升高,促进hOMF细胞的增殖和迁移。

Objective To investigate the effects of different concentrations of Tiger17 on the proliferation and migration of human oral mucosa fibroblast cells(hOMF) for different periods of time. Methods The cells were cultured and migration of hOMF cells treated with different concentrations of Tiger17 for different time were detected by CCK8 and scratch test. The expression levels of TGF-β1,ERK1/2 and p-ERK1/2 in HOMF cells were detected by Western Blot. Results The proliferative activity of hOMF was significantly higher than that of the control group at 48 h(P < 0.05) in 100 μg/mL and 200 μg/mL Tiger17;the migration rate of hOMF was significantly higher than that of the control group at 24 h and 48 h(P < 0.01) in 100 μg/mL Tiger17;different concentrations of Tiger17 acted on hOMF for 24 h and 48 h,the expression of TGF-β1 and p-Erk1/2 protein increased,and the difference was statistically significant(P < 0.05). Conclusion Tiger17 increases the expression of TGF-β1 and p-Erk1/2 in h OMF cells,and promotes the proliferation and migration of hOMF cells.