克隆杜洛克种公猪及其扩繁后代的生产性能评价

Production performance evaluation of cloned Duroc boars and their offspring

为评估克隆种公猪在生猪产业上的应用价值,进行杜洛克种公猪的克隆及扩繁试验研究。采集2头常规选育的杜洛克种公猪耳组织,建立细胞系、生产重构胚,移植到受体母猪,分娩克隆公猪并调教采精,采集精液并参配纯种杜洛克母猪,统计其生产性能,以同期普通杜洛克公猪及其后代为对照。结果显示:(1)建立了种猪耳组织细胞建系、重构胚生产、胚胎移植等技术体系;(2)移植7头代孕母猪,共产仔39头克隆杜洛克公猪,断奶存活21头,60日龄存活17头,达100 kg体重日龄测定15头,调教采精成功10头,种猪利用率为25.6%(10/39);(3)获得的克隆公猪与同期杜洛克公猪在初生重、断奶重、校正100 kg体重日龄、校正背膘厚等指标上无显著性差异;(4)获得的59头杜洛克克隆公猪后代与同期普通杜洛克公猪后代在初生重、断奶重、校正100 kg体重日龄、校正背膘等指标上无显著性差异,仅在右乳头数指标上,克隆公猪后代(6.34±0.48)显著低于普通公猪后代(6.60±0.66)。结论:建立了种猪克隆快速扩繁技术平台,但常规选育的克隆公猪及其后代生产性能与普通公猪及其后代无显著差异,需要进一步提高选择准确性,同时提高克隆效率及其存活率,才能满足克隆技术应用于终端公猪生产的产业要求。

In order to evaluate the application value of cloned boars in the pig industry, the production performance evaluation of cloned Duroc boar and their offspring were carried out. In this study, the ear tissues of two excellent conventional y bred Duroc boars were collected, the cell lines were established for reconstructed embryos. Then the reconstructed embryos were transplanted to the recipient sows, and the cloned boars were delivered. The sexualy mature boars were trained and the semen was collected and mated purebred Duroc sows through artificial insemination. Then the cloned Duroc boar and its offspring production performance were evaluated, with the common Duroc boars and their offspring as controls. The results showed that:(1) The technology platform for porcine ear tissue cell line construction, reconstructed embryo production, and embryo transfer were established;(2) 7 surrogate sows were transplanted, a total of 39 cloned Duroc boars were born, 21 of them survived at weaning time, 17 surviving at the age of 60 days, 15 at the age of 100 kg weight-age, and 10 of them were successfully trained for collecting semen, which the utilization rate of breeding pigs was 25.6%(10/39);(3) There was no significant difference between the cloned boars and the normal Duroc boars of the same period in indexes such as birth weight, weaning weight, corrected 100 kg weight-age, corrected backfat thickness, etc.;(4) There was no significant difference between the 59 Duroc cloned boar offspring and the normal Duroc boar offspring of the same period in terms of newborn weight, weaning weight, corrected 100 kg weight-age, and corrected backfat. Only in terms of the number of right nipple, cloned boars(6.34±0.48) were significantly lower than the offspring of conventional breeding boars(6.60±0.66). Conclusion: In this study, the pig cloning technology platform for rapid breeding excellent boars was established, and the production performance of cloned boars and their offspring is not significantly different from that of conventional breeding boars and their offspring. Therefore, it is necessary to further improve the selection accuracy, increase the cloning efficiency and its survival rate, to meet the industrial requirements for the application of terminal boar cloning technology.