摘要
Nanophotonics,and more specifically plasmonics,provides a rich toolbox for biomolecular sensing,since the engineered metasurfaces can enhance light–matter interactions to unprecedented levels.So far,biosensing associated with high-quality factor plasmonic resonances has almost exclusively relied on detection of spectral shifts and their associated intensity changes.However,the phase response of the plasmonic resonances have rarely been exploited,mainly because this requires a more sophisticated optical arrangement.Here we present a new phase-sensitive platform for high-throughput and label-free biosensing enhanced by plasmonics.It employs specifically designed Au nanohole arrays and a large field-of-view interferometric lens-free imaging reader operating in a collinear optical path configuration.This unique combination allows the detection of atomically thin(angstrom-level)topographical features over large areas,enabling simultaneous reading of thousands of microarray elements.As the plasmonic chips are fabricated using scalable techniques and the imaging reader is built with low-cost off-the-shelf consumer electronic and optical components,the proposed platform is ideal for point-of-care ultrasensitive biomarker detection from small sample volumes.Our research opens new horizons for on-site disease diagnostics and remote health monitoring.
基金
funded by the European Union’s Horizon 2020 research and innovation program under Grant Agreement No.644956(RAIS project)
the North Atlantic Treaty Organization’s Public Diplomacy Division in the framework of‘Science for Peace’(NATO—SPS),École Polytechnique Fédérale de Lausanne research fund,FundacióPrivada Cellex
the CERCA Programme/Generalitat de Catalunya
support from the International PhD fellowship program‘la Caixa’—Severo Ochoa@ICFO
support from the International PhD fellowship program'la Caixa'-Severo Ochoa@ICFO
support from the Spanish Ministry of Economy and Competitiveness,through the‘Severo Ochoa’Programme for Centres of Excellence in R&D(SEV-2015-0522)
project OPTO-SCREEN(TEC2016-75080-R).