摘要
Elasto-optical refractive index modulation due to photoacoustic initial pressure transients produced significant reflection of a probe beam when the absorbing interface had an appreciable refractive index difference.This effect was harnessed in a new form of non-contact optical resolution photoacoustic microscopy called photoacoustic remote sensing microscopy.A noninterferometric system architecture with a low-coherence probe beam precludes detection of surface oscillations and other phase-modulation phenomenon.The probe beam was confocal with a scanned excitation beam to ensure detection of initial pressure-induced intensity reflections at the subsurface origin where pressures are largest.Phantom studies confirmed signal dependence on optical absorption,index contrast and excitation fluence.In vivo imaging of superficial microvasculature and melanoma tumors was demonstrated with~2.7±0.5μm lateral resolution.
基金
funding from the Killam Trust(Izaak Walton Killam Memorial Scholarship)
Alberta Innovates Technology Futures
Alberta Enterprise and Advanced Education(Graduate Student Scholarship)
University of Alberta(Dissertation fellowship)
Andrew Stewart Awards and SPIE(SPIE Scholarship in Optics and Photonics)
funding from NSERC(355544-2008,375340-2009 and STPGP 396444)
the Terry-Fox Foundation
the Canadian Cancer Society(TFF 019237,TFF 019240 and CCS 2011-700718)
Alberta Innovates Health Solutions(AIHS CRIO Team Award#201201154)
The Alberta Cancer Research Institute(ACB 23728)
the Leaders Opportunity Fund of the Canada Foundation for Innovation(18472)
Alberta Advanced Education and Technology,Small Equipment Grants Program(URSI09007SEG)
Alberta Ingenuity/Alberta Innovates(scholarships for graduate and undergraduate students)
the Alberta Innovates Technology Futures Postdoctoral Fellowship.
