摘要
Photoacoustic microscopy(PAM)is an emerging imaging technology that can non-invasively visualize ocular structures in animal eyes.This report describes an integrated multimodality imaging system that combines PAM,optical coherence tomography(OCT),and fluorescence microscopy(FM)to evaluate angiogenesis in larger animal eyes.High-resolution in vivo imaging was performed in live rabbit eyes with vascular endothelial growth factor(VEGF)-induced retinal neovascularization(RNV).The results demonstrate that our multimodality imaging system can noninvasively visualize RNV in both albino and pigmented rabbits to determine retinal pathology using PAM and OCT and verify the leakage of neovascularization using FM and fluorescein dye.This work presents high-resolution visualization of angiogenesis in rabbits using a multimodality PAM,OCT,and FM system and may represent a major step toward the clinical translation of the technology.
基金
supported by a grant from the National Eye Institute 1K08EY027458(Y.M.P.)
4K12EY022299(Y.M.P.)
Fight for Sight-International Retinal Research Foundation FFSGIA16002(Y.M.P.)
unrestricted departmental support from Research to Prevent Blindness,the University of Michigan Department of Ophthalmology and Visual Sciences,and China Scholarship Council No.201606210339
the Core Center for Vision Research funded by P30 EY007003 from the National Eye Institute.
