益心泰醇提物对血管紧张素Ⅱ诱导的兔心肌成纤维细胞Bax和Bcl-2蛋白表达的影响

Effects of Yixintai Alcohol Extract on Expressions of Bax and Bcl-2 Protein in AngiotensinⅡ-induced Cardiac Fibroblasts in Rabbits

目的观察益心泰醇提物对血管紧张素Ⅱ(AngⅡ)诱导的兔心肌成纤维细胞(CFs)Bax和Bcl-2蛋白表达的影响,探讨其抗心肌纤维化的作用机制。方法提取新生兔CFs,细胞分为对照组、模型组、氯沙坦钾组和益心泰醇提物低、中、高剂量组。除对照组外,其余各组细胞予AngⅡ处理12 h建立心肌纤维化模型,再加入相应浓度药物培养一定时间,对照组加入不含AngⅡ和药物的培养基培养,CCK-8法检测CFs细胞活力,流式细胞仪检测细胞凋亡率,Western blot检测Bax、Bcl-2蛋白表达。结果与对照组比较,模型组CFs细胞活力明显升高,Bax蛋白表达显著降低,Bcl-2蛋白表达显著升高,细胞凋亡率显著降低(P<0.01);与模型组比较,益心泰醇提物低、中、高剂量组CFs细胞活力明显降低,Bax蛋白表达明显升高,Bcl-2蛋白表达明显降低(P<0.05,P<0.01),益心泰醇提物中、高剂量组细胞凋亡率明显升高(P<0.05,P<0.01)。结论益心泰醇提物可抑制AngⅡ诱导的心肌纤维化,其机制可能与抑制CFs增殖、降低细胞活力和Bcl-2蛋白表达、升高Bax蛋白表达、促进CFs凋亡有关。

Objective To observe the effects of Yixintai alcohol extract on the expressions of Bax and Bcl-2 protein in angiotensinⅡ(AngⅡ)-induced cardiac fibroblasts(CFs)in rabbits;To explore the mechanism of anti-myocardial fibrosis of Yixintai alcohol extract.Methods The CFs of newborn rabbits were extracted,and the cells were divided into control group,model group,Losartan potassium group and Yixintai alcohol extract low-,medium-,high-dosage groups.Except for the control group,cells in other groups were treated with AngⅡfor 12 h to establish a myocardial fibrosis model,and then added with the corresponding concentration of drugs for a certain period of time.The control group was cultured with medium without AngⅡor drugs.CCK-8 method was used to detect cell viability,flow cytometry was used to detect cell apoptosis rate,and Western blot was used to detect expressions of Bax and Bcl-2 protein.Results Compared with the control group,the cell viability of CFs in the model group increased,the expression of Bax protein significantly decreased,the expression of Bcl-2 protein significantly increased,and the apoptosis rate significantly decreased(P<0.01).Compared with the model group,the cell viability of CFs in Yixintai alcohol extract low-,medium-,and high-dosage groups was significantly reduced,the expression of Bax protein significantly increased,and the expression of Bcl-2 protein significantly decreased(P<0.05,P<0.01);apoptosis rate of CFs in Yixintai alcohol extract medium-and high-dosage groups significantly increased(P<0.05,P<0.01).Conclusion Yixintai alcohol extract can inhibit AngⅡ-induced myocardial fibrosis,and its mechanism may be related to inhibiting CFs proliferation,reducing cell viability and Bcl-2 protein expression,increasing Bax protein expression,and promoting CFs apoptosis.