不同饲养模式藏猪Mx1基因表达与免疫水平检测

Expression of Mx1 gene and detection of immune level in tibetan pigs under different feeding patterns

为了探索舍饲、半舍饲、放牧3种饲养模式下藏猪Mx1基因表达与免疫水平的关系,试验采用实时荧光定量PCR和ELISA方法分别对不同饲养模式下藏猪猪群Mx1基因表达和血清中α干扰素(IFN-α)水平进行了研究。结果表明:在舍饲模式下饲养的藏猪肝脏、脾脏、肺脏中Mx1基因相对表达量极显著高于半舍饲与放牧模式下饲养的藏猪(P<0.01),血清IFN-α水平显著高于半舍饲与放牧模式下饲养的藏猪(P<0.05),均表现为舍饲>半舍饲>放牧;肝脏、脾脏、肺脏中Mx1基因的相对表达量与血清IFN-α水平呈正相关,其中在肝脏和脾脏中达到显著水平(P<0.05),但舍饲藏猪健康状态仍然良好,推断可能是藏猪抗病性强,在干扰素水平上升后诱导Mx1等抗病毒蛋白基因高表达,免疫水平快速升高,对感染早期及时抑制病原起到重要作用,使猪群稳定在一个健康的水平。说明Mx1基因的表达可能与不同饲养模式下藏猪群体的免疫水平有一定关联。

In order to explore the relationship between Mx1 gene expression and immune level of Tibetan pigs under three feeding modes of house feeding, semi-house feeding and grazing, Real-time fluorescence quantitative PCR and ELISA were used to study the expression of Mx1 gene and the level of serum interferon-α(IFN-α) in Tibetan pigs under different feeding modes. The results showed that the relative expression of Mx1 gene in lever, spleen and lung of Tibetan pigs under house feeding mode was extremely significant higher than that under semi-house feeding and grazing mode(P<0.01), and the level of serum IFN-α was significantly higher than that of Tibetan pigs under semi-house feeding and grazing feeding mode(P<0.05), all of them showed house feeding> semi feeding> grazing;the relative expression of Mx1 gene was positively correlated with the level of serum IFN-α, significant levels were found in liver and spleen(P<0.05) the health status of house feeding Tibetan pigs was still good, which may be due to the strong disease resistance of Tibetan pigs, after the increase of interferon level the high expression of Mx1 and other antiviral protein genes and the rapid increase of immune level were induced, which played an important role in inhibiting the pathogen in the early stage of infection and stabilized the pigs at a healthy level. The results indicated that the expression of Mx1 gene may be related to the immune level of Tibetan pig population under different feeding patterns.