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Low sample volume origami-paper-based graphene-modified aptasensors for label-free electrochemical detection of cancer biomarker-EGFR 被引量:3

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摘要 In this work,an electrochemical paper-based aptasensor was fabricated for label-free and ultrasensitive detection of epidermal growth factor receptor(EGFR)by employing anti-EGFR aptamers as the bio-recognition element.The device used the concept of paper-folding,or origami,to serve as a valve between sample introduction and detection,so reducing sampling volumes and improving operation convenience.Amino-functionalized graphene(NH 2-GO)/thionine(THI)/gold particle(AuNP)nanocomposites were used to modify the working electrode not only to generate the electrochemical signals,but also to provide an environment conducive to aptamer immobilization.Electrochemical characterization revealed that the formation of an insulating aptamer–antigen immunocomplex would hinder electron transfer from the sample medium to the working electrode,thus resulting in a lower signal.The experimental results showed that the proposed aptasensor exhibited a linear range from 0.05 to 200 ngmL^(−1)(R^(2)=0.989)and a detection limit of 5pgmL^(−1) for EGFR.The analytical reliability of the proposed paper-based aptasensor was further investigated by analyzing serum samples,showing good agreement with the gold-standard enzyme-linked immunosorbent assay.
出处 《Microsystems & Nanoengineering》 EI CSCD 2020年第1期982-990,共9页 微系统与纳米工程(英文)
基金 This work was sponsored by the National Key Research and Development Program(2017YFA0205902) the NSFC(6196020612,61527815,61775216,61673024,and 61771452) the Key Research Programs(QYZDJ-SSW-SYS015)of Frontier Sciences,CAS the China Scholarship Council the UK Global Challenges Research Fund,the Scottish Funding Council,Engineering and Physical Sciences Research Council(EPSRC)Institutional Support Fund(Grant EP/R512813/1) as well by EPSRC(grants EP/K027611/1 and EP/R01437X/1 also supported by the National Institute for Health Research).
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