肿瘤坏死因子-α诱导对膀胱癌细胞株NF-κB信号通路分子表达及细胞增殖水平的影响

Effects of tumor necrosis factor-αinduction on molecular expression of NF-κB signaling pathway and cell proliferation level in bladder cancer cell lines

目的探讨肿瘤坏死因子-α(TNF-α)诱导对膀胱癌细胞株细胞核因子-κB(NF-κB)通路分子表达及细胞增殖水平的影响,为临床提供参考,以提高膀胱癌的治疗水平。方法选取膀胱癌细胞株T24、5637作为研究对象,根据处理的情况分为对照组和TNF-α处理组,其中对照组未采用TNF-α处理,TNF-α组经TNF-α处理,比较TNF-α处理对不同膀胱癌细胞株RelA、RelB基因的表达情况及膀胱癌细胞增殖活性的影响。结果 TNF-α处理T24细胞株后,TNF-α处理组的RelA、RelB基因表达均高于对照组(P<0.05);TNF-α处理5637细胞株后,RelA基因表达水平在作用3 h后明显上升(P<0.05),但是RelB基因表达水平与对照组相比差异无统计学意义(P>0.05)。对照组、TNF-α处理组T24和5367细胞株的存活率相比,差异均无统计学意义(P>0.05)。结论 TNF-α可诱导膀胱癌细胞NF-κB信号通路RelA、RelB基因的表达,对于细胞增殖的活性尽管有一定的影响,但是不明显。

Objective To investigate the effect of tumor necrosis factor-α(TNF-α)induction on the molecular expression of nuclear factor-κB(NF-κB)pathway and cell proliferation level in bladder cancer cell lines so as to provide reference for clinic and improve the treatment level of bladder cancer.Methods Bladder cancer cell lines T24 and 5637 were selected as the research objects and divided into the control group and TNF-αtreatment group according to the treatment conditions.The control group was not treated with TNF-α,and the TNF-αgroup was treated with TNF-α.The effects of TNF-αtreatment on the expression levels of RelA and RelB in different bladder cancer cell lines and the cell proliferation activities were compared between the two groups.Results After TNF-αtreatment in T24 cell lines,the levels of RelA and RelB gene expression in the TNF-αtreatment group were higher than those in the control group(P<0.05).After TNF-αtreatment in 5637 cell lines,the RelA gene level after 3 h of treatment was significantly increased(P<0.05),but the RelB gene level had no statistically significant difference compared with that in the control group(P>0.05).There was no statistically significant difference in the survival rate of T24 and 5367 cell lines between the control group and TNF-αtreatment group(P>0.05).Conclusion TNF-αcan induce the expression of the RelA and RelB genes in the NF-κB signaling pathway of bladder cancer cells,although which has a certain influence on the cellular proliferation activity,but which is unobvious.