Low-cost and cleanroom-free fabrication of microneedles

We present a facile,low-cost and cleanroom-free technique for the fabrication of microneedles using molds created by laser ablation.Microneedle mold with high aspect ratios is achieved on acrylic sheet by engraving a specific pattern of crossover lines(COL)using CO2 laser cutter.Ablating COL pattern on the acrylic sheet creates a sharp conical shape in the center of the design.We have shown that a variety of microneedle shapes with different heights and tip angles can be easily achieved by changing the number and the length of the COL.Polydimethylsiloxane(PDMS)microneedles were fabricated by casting the PDMS on the mold.The resulted PDMS microneedles are oxygen plasma treated and then silanized.Another PDMS layer is casted on PDMS microneedles and detached after curing.The silanization prevents those two layers of PDMS from bonding to each other and makes them easily detachable.After detachment of the PDMS mold of microneedles,the mold is used to fabricate degradable polyvinyl alcohol microneedle patch suitable for transdermal drug delivery.The release kinetics of the needles are also shown and discussed in order to prove the applicability of the needles.