水稻褐飞虱若虫和成虫褪黑素含量测定及比较

HPLC-MS/MS Determination of Melatonin in Brown Planthopper at Growth Stages

【目的】明确褪黑素在褐飞虱各发育阶段的含量情况。【方法】通过高效液相色谱-串联质谱技术(HPLCMS/MS)对1~5龄若虫和两种翅型雌、雄成虫体内褪黑素含量进行测定和比较。【结果】褐飞虱若虫期褪黑素含量在1~2龄若虫时期最低,平均为378.5 pg·g^(-1)。3龄若虫褪黑素含量最高,为856.6 pg·g^(-1)。4龄若虫褪黑素含量下降,为810.7 pg·g^(-1),与3龄无显著差异。5龄若虫褪黑素含量下降显著,为574.5 pg·g^(-1)。褐飞虱成虫褪黑素含量与性别显著相关,但受翅型影响不明显。雌、雄成虫间的褪黑素水平有极显著差异,长、短翅雄性成虫的褪黑素含量分别为978.3 pg·g^(-1)和969.6 pg·g^(-1),而长、短翅雌性的褪黑素含量仅为76.1 pg·g^(-1)和176.8 pg·g^(-1),甚至低于1~2龄若虫时期。【结论】初步明确了褐飞虱各发育阶段体内褪黑素含量变化特点,为后续研究褐飞虱褪黑素功能及机理奠定基础。

【Objective】Melatonin contents in brown planthopper,Nilaparvata lugens(Stål),at various developmental stages were determined.【Method】HPLC-MS/MS was used to measure the melatonin contents in the 1st to the 5^(th) instar nymphs as well as female and male macropterous and brachypterous adults of the insect.【Result】At nymph stage,N.lugens had the lowest average melatonin content of 378.5 pg·g^(-1)in the 1st and the 2nd instars,the highest of 856.6 pg·g^(-1)in the 3rd instar,810.7 pg·g^(-1)in the 4th instar,and 574.5 pg·g^(-1)in the 5th instar.Adult N.lugens differed significantly on melatonin contents between male and female but not between the macropterous and brachypterous wing types.The male of macropterous and brachypterous wing types contained 978.3 pg·g^(-1)and 969.6 pg·g^(-1)melatonin,respectively,which were extremely significantly higher than the female counterparts at 76.1 pg·g^(-1)and 176.8 pg·g^(-1),respectively.【Conclusion】The melatonin contents in N.lugens as nymphs and adults were determined.