一种新型载液细胞培养和观察装置的设计及其应用

A New Liquid-carrying Cell Culture and Observation Device and Its Application

设计了一种新型玻底载液装置,实现了低成本的细胞培养与观测。采用下载器支撑载物玻片,硅胶垫密封上载器和载物玻片,既实现了装置稳定的载液功能,又方便装置拆卸以进行灭菌和更换零件,实现器件的重复使用。经过细胞培养、显微成像、灭菌等实验证明,该载液观察装置满足激光共聚焦显微镜承载厚度<0.17 mm的成像要求,能够直接用于哺乳动物细胞长时间培养并进行观测。它具有可装载于普通载玻片支架、可重复使用、成本低的特点。

With respect to the defects of conventional laser confocal microscope observation devices in cell culture,high temperature and pressure sterilization and high cost,we developed a liquid carrying device,which allows low-cost cell culture and observation.The sample-loading glass slide is supported by the down-holding device.The up-holding device and sample-loading glass slide are sealed by a silica gel pad.Herein,the device not only allows stable liquid carrying,but also realizes easy disassembly for sterilization,part replacement and reuse purposes.We have shown by cell culture,microscopic imaging and sterilization experiments that the device meets the imaging requirement that the load distance of laser confocal microscope should be less than 0.17 mm.The liquid carrying device is featured by its capacity for long-time mammalian cell culture and observation,suitability for being loaded on conventional microscope slide scaffold,reusability and low cost.