高效液相色谱法同时测定和肝利胆颗粒中3种黄酮类成分含量

Simultaneous Determination of Three Flavonoids in Hegan Lidan Granules by HPLC

目的建立同时测定和肝利胆颗粒中柚皮苷、新橙皮苷、黄芩苷3种黄酮类成分含量的高效液相色谱法。方法色谱柱为Agilent Zorbax SB C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.2%磷酸溶液(20∶80,V/V),流速为1.0 m L/min,检测波长为283 nm,柱温为20℃,进样量为10μL。结果柚皮苷、新橙皮苷、黄芩苷的进样量分别在0.039 98~0.799 60μg,0.039 94~0.798 80μg,0.099 58~1.991 60μg范围内与峰面积线性关系良好(r≥0.999 5,n=6);精密度、重复性、稳定性试验的RSD均不超过2.0%(n=6);平均回收率分别为101.26%,98.74%,98.08%,RSD分别为1.21%,0.78%,1.26%(n=6)。结论该方法操作简便,结果准确,重复性好,可用于同时测定和肝利胆颗粒中柚皮苷、新橙皮苷、黄芩苷3种黄酮类成分的含量。

Objective To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of three flavonoids(naringin,neohesperidin and baicalin)in Hegan Lidan Granules.Methods The chromatographic column was Agilent Zorbax SB C18 column(250 mm×4.6 mm,5μm),t he mobile phase was acetonitrile-0.2%phosphoric acid solution(20∶80,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 283 nm,the column temperature was 20℃,and the injection volume was 10μL.Results The linear ranges of naringin,neohesperidin and baicalin were 0.03998-0.79960μg,0.03994-0.79880μg,0.09958-1.99160μg,respectively(r≥0.9995,n=6).The RSDs of precision,repeatability and stability tests were less than 2.0%(n=6).The average recoveries of naringin,neohesperidin and baicalin were 101.26%,98.74%,98.08%with RSDs of 1.21%,0.78%,1.26%(n=6),respectively.Conclusion The method is simple,accurate and reproducible,which can be used for the simultaneous determination of three flavonoids(naringin,neohesperidin and baicalin)in Hegan Lidan Granules.