Weel蛋白在浆液性卵巢腺癌组织中的表达及对患者预后的影响

The expression of wee1 in serous ovarian cancer and its influence on prognosis of patients

目的探讨wee1蛋白在浆液性卵巢腺癌组织中的表达及对患者预后的影响。方法收集2013年1月至2015年1月重庆市北碚区中医院及山东医学高等专科学校附属医院诊治的50例浆液性卵巢腺癌患者的组织和20例卵巢良性肿瘤患者的组织作为研究对象。采用实时荧光定量PCR(RT-PCR)和免疫组化法检测wee1表达情况,同时分析weel蛋白的表达与卵巢癌临床病理参数及预后的关系。结果RT-PCR结果显示,wee1在浆液性卵巢腺癌组织中高表达,而在卵巢良性肿瘤中低表达,差异具有统计学意义(P<0.05)。免疫组化法检测结果显示,wee1主要表达在细胞核中,50例浆液性卵巢腺癌组织中有38例呈阳性表达,wee1的表达与浆液性卵巢腺癌病理分级(低vs.中,P<0.05;中vs.高,P<0.05)、临床分期(Ⅰvs.Ⅲ,P<0.05;Ⅱvs.Ⅲ,P<0.05)和淋巴结转移(P<0.05)有关,而与肿瘤大小和患者年龄无关(P>0.05)。Wee1蛋白阳性表达的浆液性卵巢腺癌患者生存率为28.95%(11/38),明显低于阴性表达的75.00%(9/12),差异具有统计学意义(P<0.05)。结论Wee1蛋白在浆液性卵巢腺癌组织中高表达,临床分期、病理分级越高,Wee1的表达水平越高。而且Wee1蛋白的高表达很可能促进癌细胞的淋巴结转移,对患者的生存预后也具有参考价值。

Objective To investigate the expression of wee1 in serous ovarian cancer and its influence on prognosis of patients. Methods Tissue samples from 50 patients with serous ovarian adenocarcinoma and 20 patients with benign ovarian tumor treated in the Chinese Traditional Medicine Hospital of Beibei District and Affiliated Hospital of Shandong Medical College from January 2013 to January 2015 were collected as the research objects. Real-time quantitative PCR(RT-PCR) and immunohistochemistry were used to detect the expression of Wee1, and the relationship between Weel protein expression and clinicopathological parameters and prognosis of ovarian cancer was analyzed. Results RT-PCR results showed that the expression of WEE1 was high in serous ovarian adenocarcinoma tissues, while low in benign ovarian tumors, with statistical significance(P<0.05). The results of immunohistochemical analysis showed that wee1 was mainly expressed in the nucleus, and 38 of the 50 cases of serous ovarian adenocarcinoma were positively expressed. The expression of wee1 was correlated with the pathological grade of serous ovarian adenocarcinoma(low vs. medium, P<0.05;medium vs. high, P<0.05), clinical stage(Ⅰ vs. Ⅲ, P<0.05;Ⅱ vs. Ⅲ, P<0.05) was associated with lymph node metastasis(P<0.05), but not with tumor size or patient age(P>0.05). The survival rate of patients with serous ovarian adenocarcinoma with positive Wee1 protein expression was 28.95%(11/38), significantly lower than that with negative expression of 75.00%(9/12), and the difference was statistically significant(P<0.05). Conclusions Wee1 protein was highly expressed in serous ovarian adenocarcinoma tissues, and the higher the clinical stage and pathological grade, the higher the expression level of Wee1 protein. Moreover, the high expression of WEE1 protein is likely to promote lymph node metastasis of cancer cells, and it is also of reference value for the survival prognosis of patients.