米诺环素对腹透管金黄色葡萄球菌生物膜作用体外研究

Effect of minocycline on biofilm of S.aureus in peritoneal canal in vitro

目的研究体外环境下,腹透管表面金黄色葡萄球菌生物膜受米诺环素的影响。方法腹透液/LB液-腹透管系统行生物膜(BF)培养,建立金黄色葡萄球菌BF体外模型;试管二倍稀释法测定米诺环素最低抑菌浓度(MIC);将不同浓度米诺环素分别作用早期BF和成熟BF,连续稀释法进行各组几个作用时间段的存活菌落计数,银染法快速鉴定,扫描电镜观察BF变化。结果无论对早期BF还是成熟BF,1MIC、2MIC浓度米诺环素作用24 h后腹透管表面BF结构体积较空白对照组明显减小,且BF内活菌数减少,差异有统计学意义(P <0.05)。结论米诺环素在体外可破坏金黄色葡萄球菌已形成BF,并对BF内活菌数有协同杀菌作用。

Objective To study the effect of minocycline on S. aureus biofilm on the surface of peritoneal canal in vitro. Methods The model of S. aureus BF was established by biofilm culture in peritoneal dialysis solution/LB solutionperitoneal dialysis system. Minocycline minimum inhibitory concentration( MIC) was determined by test tube double dilution method. The early BF and mature BF were treated with different concentrations of minocycline,and the surviving colonies were counted by continuous dilution method at several time periods in each group. The BF changes was observed by silver staining method. Results For both early BF and mature BF,the volume of BF structure on the surface of peritoneal dialysis tube after treatment with 1 MIC and 2 MIC minocycline for 24 h were significantly lower than that of blank control group,and the number of viable bacteria in BF was reduced,the difference were statistically significant( P < 0. 05). Conclusion Minocycline can destroy the BF formed by S. aureus in vitro,and has synergistic bactericidal effect on the number of viable bacteria in BF.