复合共基质对菌群脱色性能及群落结构的影响

Effect of Compound Co-substrates on Decolorization Performance and Community Structure of Bacteria Flora

文章将4种不同复合共基质葡萄糖+蔗糖、葡萄糖+蛋白胨、蔗糖+蛋白胨、蛋白胨+牛肉膏,添加到混合菌群对活性黑5的脱色体系,探讨不同复合共基质对活性黑5生物脱色效果的影响,采用Miseq高通量测序技术分析脱色菌群对不同复合共基质的响应差异性。研究结果表明,蛋白胨+牛肉膏复合共基质为最佳复合共基质,在其存在条件下,脱色菌群对活性黑5(100 mg/L,48 h)的脱色率达到69.84%。Miseq高通量测序结果表明,菌群的脱色效果与微生物多样性密切相关,且在各微生物分类学水平上,菌群群落结构均呈现出较大差异。变形菌门(Proteobacteria)是所有样品的优势菌种,而厚壁菌门(Firmicutes)在最佳复合共基质蛋白胨+牛肉膏存在条件下占较大比例。另外,推测菌种Enterococcus、Lactococcus、Escherichia-Shigella可能在脱色菌群降解活性黑5过程中扮演重要角色,且复合共基质蛋白胨+牛肉膏可以为它们的生长代谢提供良好的环境。研究结果可为今后利用不同复合共基质强化微生物脱色降解实际印染废水,以及可培养功能菌种的指导筛选奠定理论基础。

Four different compound co-substrates including glucose+sucrose,glucose+peptone,sucrose+peptone,and peptone+beef extract were added into the mixed bacteria flora decolorization system of Reactive Black 5,respectively,to explore the effects of compound co-substrates on the biological decolorization of Reactive Black 5.Miseq Illumina sequencing technology was used to analyze the response differences of decolorized bacteria flora to different compound co-substrates.The results showed that peptone+beef extract was the best compound co-substrate.In the presence of it,the decolorization rate of the decolorizing bacteria flora on Reactive Black 5(100 mg/L,48 h)reached 69.84%.Miseq Illumina sequencing results reviewed that the decolorization effect of the bacteria flora was closely related to its microbial diversity,and the microbial community structure exhibited a large difference at each microbial taxonomic level.Proteobacteria were the predominant bacteria in all samples,while Firmicutes accounted for a large proportion in the presence of the best compound co-substrate peptone+beef extract.In addition,it was speculated that Enterococcus,Lactococcus,and Escherichia-Shigella might play important roles in the degradation of Reactive Black 5 by decolorizing bacteria flora,and the compound co-substrate peptone+beef extract could provide a good environment for their growth and metabolism.The study lays a theoretical foundation for the future use of different compound co-substrates to enhance microbial decolorization and degradation of actual printing and dyeing wastewater,as well as the guidance and screening of culturable functional bacteria.