癫癎发作急性期脑脊液IL-17A及其相关炎症因子水平变化的临床研究

Changes of Interleukin-17A and Related Inflammatory Factors Levels in the Cerebrospinal Fluid during the Acute Phase of Seizures

目的探讨癫癎急性发作期及发作间期白细胞介素17A (IL-17A)及其相关炎症因子在脑脊液中的含量变化。方法收集癫癎患者27例,依据2017年ILAE癫癎分类标准分为癫癎急性发作组(17例)、癫癎发作间期组(10例),同期选择14例炎性多发性神经根神经炎患者作为对照组。检测各组脑脊液常规、生化,采用蛋白芯片检测脑脊液IL-17A及其相关炎症因子水平(IL-1β、IL-6、IL-12、IFN-γ、GM-CSF)。结果急性发作组脑脊液IL-17A、IL-10水平较发作间期组显著增高,急性发作组IL-8水平较对照组显著升高。急性发作组中短期复发患者脑脊液IL-17A、IL-6水平均较无复发患者显著升高。结论癫癎急性发作期患者脑脊液IL-17A、IL-10、IL-8水平升高,而癫癎复发患者脑脊液IL-17A、IL-6升高,值得进一步深入研究。

Aim To explore the changes of levels of IL-17 A and related inflammatory factors in cerebrospinal fluid during acute seizures and inter-seizure.Methods Twenty-seven patients with confirmed epilepsy were enrolled and divided into an acute epilepsy group and an inter-seizure group.Fourteen patients with inflammatory polyneuritis were served as a control group.The patient’s cerebrospinal fluid was retained to detect the number of white blood cells,protein,glucose,chloride in the cerebrospinal fluid.And protein chips were used to detect the content of IL-17 A and related inflammatory factors(IL-1β、IL-6、IL-12、IFN-γ、GM-CSF)in the cerebrospinal fluid.Results The levels of IL-17 A and IL-10 in cerebrospinal fluid in the acute epilepsy group were significantly higher than those in the inter-seizure group.The level of IL-8 in cerebrospinal fluid in patients with acute epilepsy was higher than that of the control group.The levels of IL-17 A and IL-6 in acute cerebrospinal fluid in the recurrence group were higher than those in patients without recurrence.Conclusion IL-17 A,IL-10,IL-8 were increased in the cerebrospinal fluid of patients with acute phase of seizure,and whether the levels of cerebrospinal fluid IL-17 A and IL-6 are related to epileptic seizures are needed to study further.