基于LC-MS技术的慢性萎缩性胃炎大鼠血清标志物研究

Study on serum markers of chronic atrophic gastritis rats based on LC-MS technique

目的:基于LC-MS技术筛选慢性萎缩性胃炎(CAG)大鼠血清差异代谢物及代谢通路,寻找CAG潜在生物标志物,揭示发病机制。方法:采用MNNG联合氨水自由饮用配合饥饱失常诱导CAG大鼠模型,利用LC-MS技术对空白组和模型组大鼠的血清样品进行代谢轮廓分析,结合PCA、PLS-DA和OPLS-DA等多元统计分析方法,筛选差异代谢物及代谢通路。结果:共筛选出24种血清差异代谢物,利用MetPA得出12条差异代谢通路,CAG大鼠主要参与鞘脂代谢通路。结论:鞘脂代谢通路中的二氢鞘氨醇和神经鞘氨醇可能是CAG的潜在生物标志物。

Objective:To screen the chronic atrophic gastritis(CAG)rats serum metabolites and metabolic pathways based on the technology of LC-MS,the potential biomarkers for the CAG,and to find reveal the pathogenesis.Methods:The CAG rat model was induced by free drink of MNNG and ammonia with hunger disorder,the LC-MS technique was used to on metabolic profile analysis on the control group and model group rats serum samples,combined with PCA,PLS-DA and OPLSDA,multivariate statistical analysis methods to screen differences metabolites and metabolic pathways.Results:A total of 24 serum differential metabolites were screened out and 12 differential metabolic pathways were obtained by using MetPA.The CAG rats were mainly involved in the sphingolipid metabolic pathway.Conclusion:Dihydrosphingosine and nerve sphingosine in sphingolipid metabolism pathway may be potential biomarkers of CAG.